Establishment of a new molecular model for mercury determination verified by single crystal X-ray diffraction,spectroscopic analysis and biological potentials

查看全文 作　　者：Jiapei [1,2]Gu;Feifan [1]Zhang;Ziman [1]Zheng;Xiangqian [1,2]Li;Runxuan [1]Deng;Zhan [1]Zhou;Lufang [1]Ma;Wanqiang [3]Liu;Qianming [2,4]Wang 机构地区：[1]College of Chemistry and Chemical Engineering,Henan Key Laboratory of Function-Oriented Porous Materials,Luoyang Normal University,Luoyang 471934,China;[2]Key Laboratory of Theoretical Chemistry of Environment,Ministry of Education,School of Chemistry,South China Normal University,Guangzhou 510006,China;[3]School of Chemistry and Chemical Engineering,Hunan University of Science and Technology,Xiangtan 411201,China;[4]Guangdong Provincial Key Laboratory of Optical Information Materials and Technology&Institute of Electric Paper Displays,South China Academy of Advanced Optoelectronics,South China Normal University,Guangzhou 510006,China 出　　处：《Chinese Chemical Letters》索引2021年第32卷第1期,共5页 基　　金：Guangzhou Science and Technology Plan(No.202002030325);Science and Technology Plan of Guangdong Province(No.2020A0505100055);National Natural Science Foundation of China-Guangdong Joint Funding Support(No.U1801256);Science and Technology Program of Guangzhou(No.201905001);Guangdong Provincial Key Laboratory of Optical Information Materials and Technology(No.2017B030301007);Project of Central Plains Science and TechnologyInnovation Leading Talents of Henan Province(No.204200510001)。 摘　　要：A wide variety of molecular probes have been developed for real-time analysis,but most of organic fluorophores possess small Stokes shifts and self-absorption or inner filter effect that could not be avoided.In this study,a new dicyanoisophorone-based derivative(E)-0-(4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)phenyl)diphenylphosphinothioate(λ_(ex)=405 nm,X_(em)=551 nm,denoted as ICM-S) with strong push-pull electron effect has been afforded and it exhibits red shift for absorption from 407 nm to 426 nm with distinct color change from pale yellow to deep yellow upon exposure to Hg~(2+).Moreover,an easily distinguishable fluorescence color change follows the route from green,yellow to red in the presence of Hg~(2+) over the range of 0-90 μmol/L(detection limit=137 nmol/L)can be observed by the naked eye under a UV lamp irradiation.Chlorodiphenylphosphine and sublimedsulfur are incorpo rated as re s ponsive sites and P-O bond has been cleaved upon the addition of mercu ry ions.During the recognition process,such dicyanoisophorone dye(ICM-S) has been evolved to 2-(3-(4-hydroxystyryl)-5,5-dimethylcyclohex-2-enylidene) malononitrile(ICM-OH).Clear evidences in the chemical processes can be identified via single crystal X-ray diffraction,spectroscopic analysis,photophysical studies and titration experiments.With the aim of exploring its potential in biological systems,its in vitro responses to Hg~(2+) have been evaluated in 293 T cells and the effectiveness in zebrafish model has also been verified. 关 键 词：Dicyanoisophorone Ratiometric fluorescent probe Mercury ions Cell imaging ZEBRAFISH