Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum

查看全文 作　　者：Peisen [1]Su;Lanfei [1]Zhao;Wen [1]Li;Jinxiao [1]Zhao;Jun [2]Yan;Xin [1]Ma;Anfei [1]Li;Hongwei [1]Wang;Lingrang [1]Kong

机构地区：[1]State Key Laboratory of Crop Biology,Shandong Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Tai’an 271018,China;[2]College of Information Science and Engineering,Shandong Agricultural University,Tai'an 271018,China

出　　处：《Journal of Integrative Plant Biology》索引2021年第63卷第2期,共13页

基　　金：supported by the National Key Research and Development Program of China (2016YFD0100102-2);the National Natural Science Foundation of China (31520103911, 31871610);the Transgenic Special Item of China (2016ZX08002003-002 and 2016ZX08009-003)。 摘　　要：Fusarium head blight(FHB) caused by Fusarium graminearum Schwabe(teleomorph Gibberella zeae(Schw.) Perch) results in large yield losses in annual global wheat production. Although studies have identified a number of wheat FHB resistance genes,a deeper understanding of the mechanisms underlying host plant resistance to F. graminearum is required for the control of FHB. Here, an integrated metabolomics and transcriptomics analysis of infected wheat plants(Triticum aestivum L.) enabled identification of 789 differentially accumulated metabolites, including flavonoids, phenolamides, tryptamine derivatives, and phytohormones, and revealed altered expression of more than 100 genes that function in the biosynthesis or regulation of these pathways. Our data regarding the effects of F. graminearum infection on flavonoids and auxin signaling led to follow-up experiments that showed that exogenous kaempferide and apigenin application on spikes increased wheat resistance to FHB,while exogenous auxin treatment increased FHB susceptibility. RNAi-mediated knockdown of the gene encoding the auxin receptor, Ta TIR1, increased FHB resistance. Our data supported the use of Ta TIR1 knockdown in controlling FHB. Our study provides insights on the wheat response to F.graminearum infection and its FHB resistance mechanisms while illustrating the potential of Ta TIR1 knockdown in increasing FHB resistance during crop improvement programs. 关键词：FHB metabolomics TaTIR1 TRANSCRIPTOMICS

相关文献

参考文献（55）
引证文献（11）
耦合文献（231）

参考文献(55)

引证文献(11)

耦合文献(231)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Integrated metabolo-transcriptomics and functional characterization reveals that the wheat auxin receptor TIR1 negatively regulates defense against Fusarium graminearum

参考文献(55)

引证文献(11)

耦合文献(231)

用户提示