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Expression and regulation of genes involved in the reserve starch biosynthesis pathway in hexaploid wheat (Triticum aestivum L.)

查看全文 作　　者：Yunsong [1]Gu;Shichen [1]Han;Lin [1]Chen;Junyi [1]Mu;Luning [1]Duan;Yaxuan [1]Li;Yueming [1]Yan;Xiaohui [1]Li 高影响力作者 机构地区：[1]Key Laboratory of Genetics and Biotechnology,College of Life Science,Capital Normal University,Beijing 100048,China高影响力机构 出　　处：《The Crop Journal》索引2021年第9卷第2期,共16页高影响力期刊 基　　金：This research was supported the National Program on R&D of Transgenic Plants(2016ZX08009003-004);National Natural Science Foundation of China(31571652);the Youth Innovative Research Team of Capital Normal University. 摘　　要：Reserve starch of cereal crop accounts for about 70%of grain endosperm and acts as an important human carbohydrate resource worldwide.Wheat reserve starch is synthesized by enzymatic machinery in endosperm cells.To identify genes involved in starch biosynthesis,we constructed 30 RNA-Seq libraries of 10 endosperm-development periods and performed expression and localization analyses.Of 166 endosperm-expressed homologs of starch biosynthesis-related genes,74 showed expression correlated with reserve starch accumulation,including 26 with expected subcellular distribution and higher expression than their isoforms.The key proteins SUS3,UGP1,cAGPase,and Bt1-3 formed the main metabolic pathway and contributed the major substrates for starch processing in amyloplasts.Important isoforms,key pathway proteins,and the main carbon flux toward starch formation in the reserve starch biosynthesis pathway were identified.Based on a coexpression analysis,a library of 425 transcription factors was produced to screen for common regulators.TaMYB44 had features of transcription factors and bound to TaSUT1,TaSSIIIa,TaBEIIa,TaISA1,and TaBEIIb promoters in yeast,suggesting that the gene is a pathway regulator.This study sheds light on understanding the mechanism of reserve starch biosynthesis and will be helpful for increasing starch content in wheat endosperm via biotechnological strategies. 关 键 词：BIOSYNTHESIS Reserve starch RNA-SEQ Subcellular localization Wheat
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