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Single-molecule biotechnology for protein researches

查看全文 作　　者：Xiaoyan [1,2]Zhuang;Qian [1,2]Wu;Aihui [1,2]Zhang;Langxing [1,2]Liao;Baishan [1,2,3]Fang 高影响力作者 机构地区：[1]Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;[2]The Key Lab for Synthetic Biotechnology of Xiamen City,Xiamen University,Xiamen 361005,China;[3]The Key Laboratory for Chemical Biology of Fujian Province,Xiamen University,Xiamen 361005,China高影响力机构 出　　处：《Chinese Journal of Chemical Engineering》索引2021年第34卷第2期,共13页高影响力期刊 基　　金：This work was financially supported by the National Natural Science Foundation of China(No.21978245);National Postdoctoral Program for Innovative Talents(No.BX20200197). 摘　　要：Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy conversion.These processes are carried out in a narrow space precisely and rapidly,which,no doubt,bring great difficulty for their detection and dissection.Fortunately,in recent years,the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality,which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life activities.This paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five years.We focus on not only dissecting the difference of nanopores,atomic force microscope,scanning tunneling microscope,and optical tweezers technique,but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions,electron transport,and conformational changes.Finally,the opportunities and challenges of the single-molecule technique in protein research are discussed,which provide a new door for single-molecule protein research. 关 键 词：Single-molecule protein ENZYME NANOPORE Atomic force microscope Scanning tunneling microscope Optical tweezers BIOTECHNOLOGY IMAGING
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