Loss of scleraxis leads to distinct reduction of mineralized intermuscular bone in zebrafish

查看全文 作　　者：Chunhong [1,2]Nie;Shiming [1,2]Wan;Yulong [1,2]Chen;Dejie [1,2]Zhu;Xudong [1,2]Wang;Xiaoru [1,2]Dong;Ze-Xia [1,2,3]Gao

机构地区：[1]College of Fisheries,Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture and Rural Affair/Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction,Ministry of Education,Huazhong Agricultural University,Wuhan,Hubei,430070,China;[2]Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Wuhan,430070,China;[3]Hubei Engineering Technology Research Center for Fish Breeding and Healthy Aquaculture,Wuhan,430070,China

出　　处：《Aquaculture and Fisheries》索引2021年第6卷第2期,共9页

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grant No.31872559);National Key Research and Development Program(Grant No.2018YFD0900102);Modern Agriculture Industry Technology System Construction Projects of China titled as-Staple Freshwater Fishes Industry Technology System(Grant No.CARS-46-08);Fundamental Research Funds for the Central Universities(Grant No.2662018PY035);State Key Laboratory of Developmental Biology of Freshwater Fish(2018KF003). 摘　　要：Intermuscular bones(IBs)are ossified from tendons and only occur in lower teleosts.Positive association between the regulation of scleraxis gene(scx)and tendon development gave us reasons to speculate that the scx gene may play a potential role in regulating the development of IBs.A phylogenetic analysis conducted for this study revealed potential functional differentiation between two scx orthologues,scxa and scxb.The scxa^(-/-) and scxb^(-/-) zebrafish were generated through CRISPR-Cas9 technology to study the role of scx in the IB and rib development.The results showed a significant reduction of the number of IBs in adult scxa-1^(-/-) zebrafish,with almost 70%reduction(15-25 IBs)compared to the wild type scxa-1^(+/+)zebrafish(76-80 IBs).In the scxa-1^(+/+)adults,IBs were observed in both dorsal and tail segments;however,in scxa-1^(-/-)fish IBs were observed only in the tail segment(none in the dorsal segment).Although scxa-1^(-/-) zebrafish had rib defects,the mutants were viable and fertile as adult fish.The scxb^(-/-) zebrafish had the same number of IBs and same skeletal phenotype as the wild-type fish.This suggests that only scxa has a crucial role in the IB development,and confirms functional differentiation of scx orthologues.To further clarify the molecular mechanism by which scxa affects the IB development,we conducted comparative transcriptome analysis of dorsal tissue samples of scxa-1^(+/+)(with IBs)and scxa-1^(-/-) (without IBs),and further verified the expression of key genes via qPCR.This is the first study to identify a gene that controls the amount of IBs in fish,and it provides a new sight into the effects of scxa on the molecular mechanism of IB development in fish. 关键词：Intermuscular bone SCLERAXIS CRISPR-Cas9 PHENOTYPE Gene expression Gene function

相关文献

参考文献（45）
引证文献（14）
耦合文献（79）

参考文献(45)

引证文献(14)

耦合文献(79)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Loss of scleraxis leads to distinct reduction of mineralized intermuscular bone in zebrafish

参考文献(45)

引证文献(14)

耦合文献(79)

用户提示