Simultaneous chromatographic fingerprinting and quantitative analysis of flavonoids in Pollen Typhae by high-performance capillary electrophoresis

查看全文 作　　者：Le [1]Han;Xunhong [1]Liu;Nianyun [1]Yang;Junsong [1]Li;Baochang [1]Cai;Shi [1]Cheng

机构地区：[1]Pharmaceutical College,Nanjing University of Chinese Medicine,Nanjing 210029,China

出　　处：《Acta Pharmaceutica Sinica B》索引2012年第2卷第6期,共8页

基　　金：The authors are grateful for financial support for this project as an Opening Project of the Jiangsu Key Laboratory of Chinese Medicine Processing(ZYPZ007);by the Priority Academic Program Development of Jiangsu Higher Education Institutions(ysxk-2010). 摘　　要：To evaluate the quality of Pollen Typhae as used in traditional Chinese medicine,a high-performance capillary electrophoresis(HPCE)method has been developed,validated and applied to chromatographic fingerprinting and quantitation of its eight main bioactive flavonoids(naringenin,isorhamnetin 3-0-(2^(G)-α-L-rhamnosy)-rutinoside,rhamnetin 3-0-neohesperidoside,isorhamnetin,quercetin 3-0-(2^(G)-α-L-rhamnosyl)-rutinoside,quercetin 3-O-neohesperidoside,kaempferol and quer-cetin).Fingerprinting was based on the selection of nine characteristic chromatographic peaks.In quantitative analysis,the recovery of all eight compounds was in the range 98.5-102.2% with good linearity(r^(2)>0.9919)over a relatively wide concentration range.The assay was successfully applied to the analysis of the eight bioactive flavonoids in 14 different samples.The results indicate that the assay is reproducible and precise and can be used for convenient quality assessment of Pollen Typhae. 关键词：High-performance capillary electrophoresis Pollen Typhae FINGERPRINT Simultaneous quantification FLAVONOIDS

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Simultaneous chromatographic fingerprinting and quantitative analysis of flavonoids in Pollen Typhae by high-performance capillary electrophoresis

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