Sharpening emitter localization in front of a tuned mirror

查看全文 作　　者：Hannah [1]S.Heil;Benjamin [1]Schreiber;Ralph [2]Götz;Monika [3]Emmerling;Marie-Christine [4]Dabauvalle;Georg [4]Krohne;Sven [3,5]Höfling;Martin [3]Kamp;Markus [2]Sauer;Katrin [1]G.Heinze 机构地区：[1]Rudolf Virchow Center,Research Center for Experimental Biomedicine,University of Würzburg,Josef-Schneider-Str.2,97080 Würzburg,Germany;[2]Department of Biotechnology and Biophysics,Biozentrum,University of Würzburg,Am Hubland,97074 Würzburg,Germany;[3]Technische Physik,Physikalisches Institut and Wilhelm Conrad Röntgen-Center for Complex Material Systems,University of Würzburg,Am Hubland,97074 Würzburg,Germany;[4]Division of Electron Microscopy,Biozentrum,University of Würzburg,Am Hubland,97074 Würzburg,Germany;[5]SUPA,School of Physics and Astronomy,University of St Andrews,St Andrews KY169SS,UK 出　　处：《Light(Science & Applications)》索引2018年第7卷第1期,共8页 基　　金：supported by the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)TRR 166 projects A04(to M.S.)and C06(to K.G.H.);the Rudolf Virchow Center of the University of Würzburg(H.S.H.);the Elite Network of Bavaria(ENB)with project K-BM-2013-247(to B.S.);the University of Würzburg(M.E.,M.K.,S.H.,and R.G.);the State of Bavaria(clean room facilities). 摘　　要：Single-molecule localization microscopy(SMLM)aims for maximized precision and a high signal-to-noise ratio1.Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4.Here,we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two.The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength,as experimentally demonstrated for dual-color SMLM in cells. 关 键 词：EMITTER MIRROR TUNABLE