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BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

查看全文 作　　者：Quan-Yan [1]Zhang;Kai-Di [1]Gu;Jia-Hui [1]Wang;Jian-Qiang [1]Yu;Xiao-Fei [1]Wang;Shuai [2]Zhang;Chun-Xiang [1]You;Da-Gang [1]Hu;Yu-Jin [1]Hao 高影响力作者 机构地区：[1]National Key Laboratory of Crop Biology,Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient Production,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai-An,Shandong 271018,China;[2]College of Chemistry and Material Science,Shandong Agricultural University,Tai-An,Shandong 271018,China高影响力机构 出　　处：《Horticulture Research》索引2020年第7卷第1期,共12页高影响力期刊 基　　金：supported by grants from the National Key Research and Development Program of China(2018YFD1000200);the National Natural Science Foundation of China(31972375);Ministry of Agriculture(CARS-27);Shandong Province(SDAIT-06-03). 摘　　要：As an important primary metabolite,malate plays a key role in regulating osmotic pressure,pH homeostasis,stress tolerance,and fruit quality of apple.The R2R3-MYB transcription factor(TF)MdMYB73 was identified as a protein that plays a critical role in determining malate accumulation and vacuolar acidification by directly regulating the transcription of aluminum-activated malate transporter 9(MdALMT9),vacuolar ATPase subunit A(MdVHA-A),and vacuolar pyrophosphatase 1(MdVHP1)in apple.In addition,the bHLH TF MdCIbHLH1 interacts with MdMYB73 and enhances the transcriptional activity of MdMYB73.Our previous studies demonstrated that the BTB-BACK-TAZ domain protein MdBT2 can degrade MdCIbHLH1 to influence malate accumulation and vacuolar acidification.However,the potential upstream regulators of MdMYB73 are currently unknown.In this study,we found that MdBT2 directly interacts with and degrades MdMYB73 through the ubiquitin/26S proteasome pathway to regulate malate accumulation and vacuolar acidification.A series of functional assays with apple calli and fruit showed that MdBT2 controls malate accumulation and vacuolar acidification in an MdMYB73-dependent manner.Overall,our findings shed light on the mechanism by which the BTB-BACK-TAZ domain protein MdBT2 regulates malate accumulation and vacuolar acidification by targeting MdMYB73 and MdCIbHLH1 for ubiquitination in apple.This information may help guide traditional breeding programs and fruit tree molecular breeding,and lead to improvements in fruit quality and stress tolerance. 关 键 词：MALATE ubiquitin homeostasis
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