Tomato fruit as a model for tissue-specific gene silencing in crop plants

查看全文 作　　者：Ari [1]Feder;Sarah [1,2]Jensen;Anquan [1,3]Wang;Lance [1,4]Courtney;Lesley [1]Middleton;Joyce Van [1]Eck;Yongsheng [3]Liu;James [1,4,5]J.Giovannoni

机构地区：[1]Boyce Thompson Institute for Plant Research,Cornell University,Ithaca,NY,USA;[2]Section of Plant Breeding and Genetics,Cornell University,Ithaca,NY,USA;[3]School of Biotechnology and Food Engineering,Hefei University of Technology,230009 Hefei,China;[4]Section of Plant Biology,Cornell University,Ithaca,NY,USA;[5]US Department of Agriculture–Agricultural Research Service,Robert W.Holley Center for Agriculture and Health,Ithaca,NY,USA

出　　处：《Horticulture Research》索引2020年第7卷第1期,共7页

基　　金：supported by National Science Foundation Plant Genome Program grant IOS-1855585 to JG and the US Department of Agriculture-Agricultural Research Service. 摘　　要：Use of CRISPR-Cas9(Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)-CRISPR-associated 9)-mediated genome editing has proliferated for use in numerous plant species to modify gene function and expression,usually in the context of either transient or stably inherited genetic alternations.While extremely useful in many applications,modification of some loci yields outcomes detrimental to further experimental evaluation or viability of the target organism.Expression of Cas9 under a promoter conferring gene knockouts in a tissue-specific subset of genomes has been demonstrated in insect and animal models,and recently in Arabidopsis.We developed an in planta GFP(green fluorescent protein)assay system to demonstrate fruit-specific gene editing in tomato using a phosphoenolpyruvate carboxylase 2 gene promoter.We then targeted a SET-domain containing polycomb protein,SlEZ2,previously shown to yield pleiotropic phenotypes when targeted via ^(35)S-driven RNA interference and we were able to characterize fruit phenotypes absent additional developmental perturbations.Tissue-specific gene editing will have applications in assessing function of essential genes otherwise difficult to study via germline modifications and will provide routes to edited genomes in tissues that could not otherwise be recovered when their germline modification perturbs their normal development. 关键词：otherwise SPECIFIC EDITING

相关文献

参考文献（30）
引证文献（2）
耦合文献（0）

参考文献(30)

引证文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Tomato fruit as a model for tissue-specific gene silencing in crop plants

参考文献(30)

引证文献(2)

用户提示