维普中文期刊产品整合服务

Development of two TaqMan real-time reverse transcription-PCR assays for the detection of severe acute respiratory syndrome coronavirus-2

查看全文 作　　者：Yiwei [1]Liu;Yingying [1]Wang;Xinming [1]Wang;Yan [1]Xiao;Lan [1]Chen;Li [1]Guo;Jianguo [2]Li;Lili [1,3]Ren;Jianwei [1,3]Wang 高影响力作者 机构地区：[1]National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory,Institute of Pathogen Biology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100730,China;[2]Institutes of Biomedical Sciences,Shanxi University,Taiyuan 030006,China;[3]Key Laboratory of Respiratory Disease Pathogenomics,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China高影响力机构 出　　处：《Biosafety and Health》索引2020年第2卷第4期,共6页高影响力期刊 基　　金：funded in part by the Project from the Ministry of Science and Technology in China(2020YFC0841200);the National Major Science&Technology Project for Control and Prevention of Major Infectious Diseases in China(2017ZX10103004);the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2020HY320001);the non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019PT310029);the Chinese Academy of Medical Sciences(CAMS)Innovation Fund for Medical Sciences(2016-I2M-1-014). 摘　　要：The outbreak of coronavirus disease 2019(COVID-19)in Wuhan,China,was caused by a novel coronavirus(CoV),named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The rapid detection of viral nucleic acids is critical for the early identification of infected cases.We have developed two TaqMan real-time reverse transcription-PCR assays to detect SARS-CoV-2.The designed primers target the nucleocapsid(N)and open reading frame(ORF)1b gene regions,where the probes discriminate SARS-CoV-2 from other human and animal CoVs.The sensitivities are one genomic copy per reaction for theN gene assay and ten copies for the ORF 1b gene assay.The overall linear detection ranges are 1–10^(6)and 10–10^(6)copies per reaction for the N gene assay and the ORF 1b gene assay,respectively.Surveillance of 23 suspected COVID-19 patients demonstrated that SARS-CoV-2 could be detected from 100%(23/23)and 62.5%(16/23)of clinical specimens by the N gene assay and the ORF 1b gene assay,respectively.All of the samples not detected by the ORF 1b gene assay were throat swabs,indicating a lower viral load in the upper respiratory tract and the relatively lower sensitivity of the ORF 1b gene assay.The assays developed in the present study offer alternative diagnostic tests for COVID-19. 关 键 词：SARS-CoV-2 Real-time RT-PCR NUCLEOCAPSID Open reading frame 1b
相关文献

参考文献(12)

引证文献(5)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费