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Comparative Transcriptome Analysis of Heart Tissue in Response to Hypoxia in Silver Sillago (Sillago sihama)

查看全文 作　　者：SAETAN [1]Wanida;YE [1]Minghui;LIN [1]Xinghua;LIN [1]Xiaozhan;ZHANG [1]Yulei;HUANG [1]Yang;DU [1]Tao;LI [1]Guangli;TIAN [1]Changxu 高影响力作者 机构地区：[1]Fisheries College,Guangdong Ocean University,Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species,Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding,Southern Marine Science and Engineering Guangdong Laboratory,Zhanjiang 524088,China高影响力机构 出　　处：《Journal of Ocean University of China》索引2021年第20卷第4期,共10页高影响力期刊 基　　金：This study was supported by grants from the National Natural Science Foundation of China(Nos.41706174 and 31702326);the Natural Science Foundation of Guangdong Province(No.2019A1515110619);the Department of Education of Guangdong Province(Nos.2018KQNCX111 and 2019KTSCX060);the College Students’Innovation and Entrepreneurship Project of Guangdong Province(No.CX XL2019138);the Program for Scientific Research Startup Funds of Guangdong Ocean University(No.R19026). 摘　　要：Sillago sihama,commonly known as silver sillago,is considered as an economically important fish species in China.It is sensitive to hypoxia stress in the larval stage,and the mechanism has not been understood thoroughly.In this study,we investigated the transcriptome change in heart tissues under hypoxia stress.The fish were divided into four groups,including 1 h of hypoxia(hypoxia1h,dissolved oxygen(DO)=1.5±0.1 mg L^(−1)),4h of hypoxia(hypoxia4h,DO=1.5±0.1 mg L^(−1)),4h of reoxygen(reoxygen4h,DO=8.0±0.2 mg L^(−1))after 4h of hypoxia(DO=1.5 mg L^(−1))and normoxia or control(DO=8.0±0.2 mg L^(−1))groups.The results showed that a total of 3068 genes were identified as differentially expressed genes(DEGs)based on the criteria∣log2(Fold change)∣>1.0 and adjusted P-value<0.05.A total of 7761141 and 1151 DEGs were obtained from hypoxia1h,hypoxia4h and reoxygen4h groups,respectively.The enrichment pathway analysis showed that the DEGs were significantly enriched in ribosome biogenesis in eukaryotes,retinol metabolism,DNA replication and the oxidative phosphorylation(OXPHOS)pathways.Thirteen DEGs from the RNA-seq results were validated by quantitative real-time polymerase chain reaction(qRT-PCR).These candidate genes are considered as important regulatory factors involved in the hypoxia stress response in S.sihama. 关 键 词：TRANSCRIPTOMES heart tissues hypoxia stress Sillago sihama gene expression
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