The Evaluation of Insertion and Deletion Polymorphism in Population and Personal Identification Amidst Chinese Populations

查看全文 作　　者：Hui [1]Sun;Caiyong [2,3,4]Yin;Lei [1]Shang;Chong [1]Wang;Kaiyuan [3,4]Su;Wanshui [1]Li;Feng [2]Chen;Shilin [3,4]Li 机构地区：[1]Institute of Forensic Science,Ministry of Public Security,Beijing 100038;[2]Department of Forensic Medicine,Nanjing Medical University,Nanjing,210029;[3]MOE Key Laboratory of Contemporary Anthropology,Department of Anthropology and Human Genetics,School of Life Sciences,Fudan University,Shanghai,200438;[4]Fudan-Taizhou Institute of Health Sciences,Jiangsu,225300,China 出　　处：《Journal of Forensic Science and Medicine》索引2018年第4卷第3期,共10页 基　　金：This study was financially supported by The Fundamental Research Funds for the Central Research Institutes with project number“2017JB004.” 摘　　要：For comprehensive understanding of practical application and evaluation on the power of30 commonly used InDeis(Qiagen Investigator DIPplex®kit),we captured population data from 25 Chinese populations and employed F-statistics for population genetics analysis.The results indicated that the distributions of allelic frequencies among populations were in different levels.Furthermore,the phylogeny confoiming pairwise FST distances showed that the difierentiation of majority populations were consistent with their geographic locations and historic dispersals.We conduct the comprehensive correlation analysis between FST and heterozygosity of30 InDel loci and provided strong evidence for ongoing InDei loci selection.The Fst values of 30 InDels were calculated within 25 Chinese populations,and then,these loci were characterized definitely based on their roles in population genetics or individual identification.Data indicated that 17 InDels with FST<0.01 could be utilized regarding Chinese individual identification(total discrimination power=0.999985 and cumulative matching probability=0.00000009).We comprehensively reconstructed the population structure and filled the gap of evaluating the ability of InDels in personal as well as population identification.The application of InDel loci in the forensic area would convincingly promote the development matter of forensic population identification and personal discrimination. 关 键 词：Chinese populations insertion and deletion personal Identification population identification Qiagen investigator dipplex®kit