C/EBP-β and SIRT1 regulate IL-18 expression in the proliferative phase endometria of polycystic ovary syndrome patients

查看全文 作　　者：Xiaoyu [1,2,3]Long;Honghao [1,2,3,4]Wang;Xiaohui [1,2,3]Zhu;Rong [5]Li;Yan [1,2,3]Yang;Jie [1,2,3]Qiao

机构地区：[1]Reproductive Medical Center,Department of Obstetrics and Gynecology,Peking University Third Hospital,Beijing,100191,China;[2]Key Laboratory of Assisted Reproduction,Ministry of Education,Beijing,100191,China;[3]Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology,Beijing,100191,China;[4]College of Life Sciences,Yantai University,Yantai,Shandong Province,264000,China;[5]Peking University Third Hospital,China

出　　处：《Gynecology and Obstetrics Clinical Medicine》索引2021年第1卷第2期,共6页

基　　金：This work was supported by the National Natural Science Foundation of China-Youth Foundation(grant number 81401169);National Natural Science Foundation of China-Youth Foundation(grant number 81701407);National Natural Science Foundation of China Emergency Management Project(grant number 81541015). 摘　　要：Background:Previous studies have shown that polycystic ovary syndrome(PCOS)patients suffer from low-grade chronic inflammation.Furthermore,our previous studies have confirmed that IL-18 is highly expressed in the serum and endometria of PCOS patients.Nevertheless,the mechanisms underlying IL-18 elevation remain unclear.This study aims to explore the signaling pathways that lead to IL-18 upregulation in the endometria of PCOS patients.Methods:Using the TF-Search tool,we predicted that C/EBP-βmay be a transcription factor for IL-18 and that deacetylase SIRT1 could be involved in its regulation.Subsequently,we analyzed SIRT1 and C/EBP-βin the proliferative endometria of PCOS patients and healthy control using immunohistochemistry,real-time quantitative PCR and western blot;PCOS diagnosis was based on the 2003 Rotterdam ESHRE/ASRM criteria.We further verified the interaction between C/EBP-βand IL-18 using a double luciferase assay.Results:SIRT1 and C/EBP-βgene and protein levels in the proliferative endometria of PCOS patients were significantly higher than the control group.Immunohistochemical experiments confirmed that SIRT1 was primarily expressed in the endometrial nucleus,while C/EBP-βwas predominantly expressed in the endometrial nucleus and cytoplasm.Double luciferase assay verified the interaction between C/EBP-βand IL-18.Conclusion:SIRT1 and C/EBP-βare probable IL-18 regulatory mechanism in the endometria of PCOS patients. 关键词：SIRT1 C/EBP DIAGNOSIS

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C/EBP-β and SIRT1 regulate IL-18 expression in the proliferative phase endometria of polycystic ovary syndrome patients

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