A Recessive Mutant of argonaute 1b/gsnl4 Leads to Narrow Leaf, Small Grain Size and Low Seed Setting in Rice

查看全文 作　　者：SONG [1,2]Mengqiu;RUAN [1,2]Shuang;PENG [3]Youlin;WANG [1]Zhongwei;Jahan [1]NOUSHIN;ZHANG [1]Yu;CUI [1]Yongtao;HU [1]Haitao;JIANG [1]Hongzhen;DING [1]Shilin;SHEN [1]Lan;GAO [1]Zhenyu;HU [1]Xingming;QIAN [1]Qian;GUO [1]Longbiao 机构地区：[1]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;[2]College of Agronomy,Anhui Agricultural University,Hefei 230036,China;[3]Rice Research Institute,Southwest University of Science and Technology,Mianyang 621010,China 出　　处：《Rice science》索引2021年第28卷第6期,共29页 基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31771887,31671761 and 32001491);supported by the National Natural Science Foundation of China(Grant Nos.31771887,31671761 and 32001491). 摘　　要：A gsnl4 mutant characterized by small grain size,narrow leaf and low seed-setting rate was obtained by ethyl methane sulfonate(EMS)mutagenesis of a japonica rice variety Wuyunjing 21.Genetic analysis showed that gsnl4 is a loss-of-function mutant.A single-base mutation in gsnl4 resulted in the substitution of Ser to Asn in the Piwi domain of OsAGOlb protein.CRISPR/Cas9-mediated editing of OsAGOlb yielded a mutant phenotypically resembling gsnl4.Furthermore,miRNA-Seq analysis showed that the transcript expression levels of miRNAs in the signal transduction pathways related to pollen development,leaf morphology and honnone activation were significantly different between the gsnl4 mutant and the wild type(WT)plants.Several miRNAs were downregulated,and their target genes were upregulated in gsnl4 mutants.The auxin content in the root tips of the gsnl4 mutant decreased,and the expression of most auxin-related genes was altered.In summary,GSNL4 not only regulates organ development by controlling cell division and expansion,but also plays an important role in regulating auxin transport in rice. 关 键 词：CRISPR/Cas9 NARROW SMALL