Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

查看全文 作　　者：Xiao [1]Zheng;Xiaorong [2,3]Zhang;Yingting [3]Wang;Yangxi [4,5]Liu;Yining [1]Pan;Yijia [1]Li;Man [1]Ji;Xueqin [6]Zhao;Shengbin [3,7]Huang;Qingqing [1]Yao

机构地区：[1]Institute of Advanced Materials for Nano-Bio Applications,School of Ophthalmology&Optometry,Eye Hospital,Wenzhou Medical University,270 Xueyuan Xi Road,Wenzhou,Zhejiang,325027,PR China;[2]Department of Endodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,PR China;[3]Institute of Stomatology,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,PR China;[4]Comprehensive Transplant Center,Feinberg School of Medicine,Northwestern University,USA;[5]Department of Surgery,Feinberg School of Medicine,Northwestern University,USA;[6]College of Life Science and Medicine,Zhejiang Sci-Tech University,Hangzhou,310018,PR China;[7]Department of Prosthodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China

出　　处：《Bioactive Materials》索引2021年第6卷第10期,共11页

基　　金：This work is supported by the Chinese National Natural Science Foundation of China(31600773);Zhejiang Provincial Natural Science Foundation of China(LY18C100002). 摘　　要：Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis.In present work,a nanoclay(Laponite,XLS)-functionalized 3D bioglass(BG)scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods.Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells(ADSCs)compared to the properties of the neat BG scaffold.Desferoxamine,a hypoxia mimicking agent,encourages bone regeneration via activating hypoxia-inducible factor-1 alpha(HIF-1α)-mediated angiogenesis.GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation.Furthermore,in vitro data demonstrated increased HIF-1αand vascular endothelial growth factor(VEGF)expressions on human adipose mesenchymal stem cells(ADSCs).Moreover,BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs.Most importantly,our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model.Therefore,we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF,but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration. 关键词：3D bioglass scaffold HYPOXIA Angiogenesis OSTEOGENESIS Endogenous bone regeneration

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Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

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