New design to remove leukocytes from platelet-rich plasma(PRP)based on cell dimension rather than density

查看全文 作　　者：Subhaini [1,2]Jakfar;Tzu-Chieh [1]Lin;Shinn-Chih [3]Wu;Yao-Horng [4]Wang;Yu-Jun [1]Sun;Minal [1]Thacker;Li-Xin [5]Liu;Feng-Huei [1,6]Lin

机构地区：[1]Institute of Biomedical Engineering,National Taiwan University,Taipei,106,China;[2]Dentistry Faculty,Syiah Kuala University,Darussalam,23111,Banda Aceh,Indonesia;[3]Department of Animal Science and Technology,National Taiwan University,Taipei,China;[4]Department of Nursing,Yuanpei University of Medical Technology,306,Yuanpei Street,Hsinchu,300,China;[5]School of Materials Science and Engineering,Center of Functional Biomaterials,Key Laboratory of Polymeric Composite Materials and Functional Materials of Education,GD Research Center for Functional Biomaterials Engineering and Technology,Sun Yat-Sen Unversity,Guangzhou,510275,China;[6]Institute of Biomedical Engineering and Nanomedicine,National Health Research Institutes,Miaoli,350,China

出　　处：《Bioactive Materials》索引2021年第6卷第10期,共13页

基　　金：This work was supported by the Indonesia Endowment Fund for Education(LPDP)within the ministry of Finance,Indonesia(grant number PRJ-317/LPDP.3/2016). 摘　　要：Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue repair.Although many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP kits.The work of commercial PRP kits is based on the density of blood elements.However,the blood elements are very close in density which makes the separation challenging.Therefore,the mentioned commercialized kits are generally contaminated by leucocytes and erythrocyte.In this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge tube.The home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage potentials.The concentration in PRP prepared by group H2M was relatively stable and far above average.All the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell migration.We believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers. 关键词：Platelet-rich plasma(PRP) Filtered-tube design Homemade design Cut-off pore size PLATELETS Growth factors

相关文献

参考文献（52）
引证文献（1）
耦合文献（37）

参考文献(52)

引证文献(1)

耦合文献(37)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

New design to remove leukocytes from platelet-rich plasma(PRP)based on cell dimension rather than density

参考文献(52)

引证文献(1)

耦合文献(37)

用户提示