A cell wall-localized NLR confers resistance to Soybean mosaic virus by recognizing viral-encoded cylindrical inclusion protein.

查看全文 作　　者：Jinlong [1]Yin;Liqun [1]Wang;Tongtong [1]Jin;Yang [1]Nie;Hui [1]Liu;Yanglin [2]Qiu;Yunhua [1]Yang;Bowen [1]Li;Jiaojiao [2]Zhang;Dagang [1]Wang;Kai [1]Li;Kai [2]Xu;Haijian [1]Zhi

机构地区：[1]National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement,Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture,Nanjing Agricultural University,Nanjing,China;[2]Jiangsu Key Laboratory for Microbes and Functional Genomics,College of Life Sciences,Nanjing Normal University,Nanjing,China

出　　处：《Molecular Plant》索引2021年第14卷第11期,共20页

基　　金：This work was supported by the National Natural Science Foundation of China(31571690,31770164);the Fundamental Research Funds for the Central Universities(KYT201801);Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT_17R55);the National Soybean Industrial Technology System of China(CARS-004);Jiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP),the National Key R&D Program of China(2017YFD0101501);the Natural Science Foundation of Jiangsu Province(BK20180039). 摘　　要：Soybean mosaic virus (SMV) causes severe yield losses and seed quality reduction in soybean (Glycine max) production worldwide. Rsc4 from cultivar Dabaima is a dominant genetic locus for SMV resistance, and its mapping interval contains three Nucleotide-binding domain Leucine-rich Repeat containing (NLR) candidates (Rsc4-1, Rsc4-2, and Rsc4-3). The NLR-type resistant proteins were considered as important intracellular pathogen sensors in the previous studies. In this research, based on transient expression assay in Nicotiana benthamiana leaves, we found that the longest transcript of Rsc4-3 is sufficient to induce resistance response to SMV;and CRISPR/Cas9-mediated Rsc4-3 knockout in resistant cultivar Dabaima compromised the resistance. These indicate that Rsc4-3 confers resistance to SMV. Interestingly, Rsc4-3 encodes a cell wall localized NLR-type resistant protein (Rsc4-3). The internal polypeptide region responsible for apoplastic targeting of Rsc4-3 and the putative palmitoylation sites on the N-terminus are essential for the resistance response. Furthermore, we showed that viral-encoded cylindrical inclusion (CI) protein partially localizes to the cell wall and can interact with Rsc4-3. Virus-driven or transient expression of CI protein of avirulent SMV strains is enough to induce resistance response in the presence of Rsc4-3, suggesting that CI is the avirulent gene for Rsc4-3 mediated resistance. Our work exhibited a case of NLR recognizing virus in the apoplast and provided a simple and effective method for identifying resistant genes against SMV infection. 关键词：Cell wall NLR Rsc4 Soybean mosaic virus PALMITOYLATION resistance gene

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A cell wall-localized NLR confers resistance to Soybean mosaic virus by recognizing viral-encoded cylindrical inclusion protein.

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