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Quantitative evaluation of DNA damage caused by atmospheric and room-temperature plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding

查看全文 作　　者：Yuting [1]Huang;Liyang [2]Wang;Xue [3]Zhang;Nan [1]Su;Heping [4]Li;Yoshimitsu [5]Oda;Xinhui [1,6,7,8]Xing 高影响力作者 机构地区：[1]Key Laboratory for Industrial Biocatalysis,Ministry of Education of China,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China;[2]Biobreeding Research Center,Wuxi Research Institute of Applied Technologies,Tsinghua University,Wuxi 214072,China;[3]Department of Entomology,College of Plant Protection,China Agricultural University,Beijing 100193,China;[4]Department of Engineering Physics,Tsinghua University,Beijing 100084,China;[5]Institute of Life and Environmental Sciences,Osaka Shin-Ai College,Tsurumi,Tsurumi-ku,Osaka 5380052,Japan;[6]Centre for Synthetic and Systems Biology,Tsinghua University,Beijing 100084,China;[7]Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China;[8]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518055,China高影响力机构 出　　处：《Chinese Journal of Chemical Engineering》索引2021年第34卷第11期,共6页高影响力期刊 基　　金：National Key Research and Development Project of China(2016YFD0102106);National Key Scientific Instrument and Equipment Project of National Natural Science Foundation of China(21627812). 摘　　要：Mutagenesis is an important technique for microbial mutation breeding.As the source of mutations,DNA damage extent is a key indicator for the effectiveness of mutagenesis.Therefore,a rapid and easy DNA damage quantification method is required for the comparison of mutagenesis effects and development of mutagenesis tools.Here,we used the umu-microplate test system to quantitatively compare the DNA damage strength caused by atmospheric and room-temperature plasma(ARTP)and other traditional mutagenesis methods including:ultraviolet radiation(UV),diethyl sulfate(DES)and 4-nitroquinoline-1-oxide(4-NQO).The test strain of Salmonella typhimurium TA1535/pSK1002 was used to monitor the time-course profile of b-galactosidase activity induced by DNA damage caused by different mutagenesis methods using a microplate reader.The umu-microplate test results showed that ARTP caused higher extent of DNA damage than UV and chemical mutagens,which agrees well with the result obtained by SOS-FACS-based quantification method as reported previously.This umu-microplate test is accessible for broad researchers who are lack of the expensive FACS instruments and allows the quick quantitative evaluation of DNA damage among living cells for different mutagenesis methods in the study of the microbial mutation breeding. 关 键 词：ARTP mutagenesis umu-Microplate test Biological engineering Cell engineering BIOTECHNOLOGY
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