An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya

查看全文 作　　者：Decai [1,2]Tuo;Pu [1,2]Yan;Guangyuan [1]Zhao;Hongguang [3]Cui;Guopeng [4]Zhu;Yang [1,4]Liu;Xiukun [1,4]Yang;He [1,4]Wang;Xiaoying [1,2]Li;Wentao [1,2,4,5]Shen;Peng [1,2,4,5]Zhou

机构地区：[1]Key Laboratory of Biology and Genetic Resou rces of Tropical Crops,Min ist ry of Agriculture and Rural Affairs&Institute of Tropical Bioscienee and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,571101 Haikou,China;[2]Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources&Institute for Tropical Agricultural Resources,Chinese Academy of Tropical Agricultural Sciences,571101 Haikou,China;[3]College of Plant Protection,Hainan University,570228 Haikou,China;[4]College of Horticulture,Hainan University,570228 Haikou,China;[5]Hainan Key Laboratory of Tropical Microbe Resources,571101 Haikou,China

出　　处：《Horticulture Research》索引2021年第8卷第1期,共12页

基　　金：This work was supported by the Hainan Provincial Natural Science Foundation of China(2019RC298 and 2018CXTD343);the National Natural Science Foundation of China(32072390);the Central Public Interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(grant no.19CXTD-33)。 摘　　要：Papaya(Carica papaya L.)is regarded as an excellent model for genomic studies of tropical trees because of its short generation time and its small genome that has been sequenced.However,functional genomic studies in papaya depend on laborious genetic transformations because no rapid tools exist for this species.Here,we developed a highly efficient virus-induced gene silencing(VIGS)vector for use in papaya by modifying an artificially attenuated infectious clone of papaya leaf distortion mosaic virus(PLDMV;genus:Potyvirus),PLDMV-E,into a stable Nimble Cloning(NC)-based PLDMV vector,pPLDMV-NC,in Escherichia coli.The target fragments for gene silencing can easily be cloned into pPLDMV-NC without multiple digestion and ligation steps.Using this PLDMV VIGS system,we silenced and characterized five endogenous genes in papaya,including two common VIGS marker genes,namely,phytoene desaturase,Mg-chelatase H subunit,putative GIBBERELLIN(GA)-INSENSITIVE DWARF1A and 1B encoding GA receptors;and the cytochrome P450 gene CYP83B1,which encodes a key enzyme involved in benzylglucosinolate biosynthesis.The results demonstrate that our newly developed PLDMV VIGS vector is a rapid and convenient tool for functional genomic studies in papaya. 关键词：species PAPAYA VIGS

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An efficient papaya leaf distortion mosaic potyvirus vector for virus-induced gene silencing in papaya

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