Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo

查看全文 作　　者：ianbing [1]Hu;Qiang [1]Xu;Chenchen [1]Liu;Binghao [2]Liu;Chongling [2]Deng;Chuanwu [2]Chen;Zhuangmin [3]Wei;Muhammad Husnain [1]Ahmad;Kang [1]Peng;Hao [1]Wen;Xiangling [4]Chen;Peng [5]Chen;Robert [1]MLarkin;Junli [1]Ye;Xiuxin [1]Deng;Lijun [1]Chai

机构地区：[1]Key Lab oratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,People's Republic of China;[2]Guangxi Engineering Research Center of Citrus Breeding and Culture,Guangxi Academy of Specialty Crops,Guilin 541004,People's Republic of China;[3]Guangxi Subtropical Crops Research Institute,Nanning 530001,People's Republic of China;[4]Horticulture Research Institute,Guangxi Academy of Agriculture Sciences,Nanning Investigation&Experiment Station of South Subtropical Fruit Trees,Ministry of Agriculture,Nanning 530007 Guangxi,People's Republic of China;[5]Horticultural Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,People's Republic of China

出　　处：《Horticulture Research》索引2021年第8卷第1期,共15页

基　　金：This research was financially supported by the National Key Research and Development Program of China(grant no.2018YFD1000107);the National Natural Science Foundation of China(grant nos.31772259,31630065,and 31521092);the Fundamental Research Funds forthe Central Univer sities(grant no.2662019PY044);the China Agriculture Research System of MOF and MARA and the Hubei Provincial Natural Science Foundation of China(2020CFB532). 摘　　要：Self-incompatibility(SI)substantially restricts the yield and quality of citrus.Therefore,breeding and analyzing selfcompatible germplasm is of great theoretical and practical signi ficance for citrus.Here,we focus on the mechanism of a self-compatibility mutation in‘Guiyou No.1'pummelo(Citrus maxima),which is a spontaneous mutant of‘Shatian’pummelo(Citrus maxima,self-incompatibility).The rate of fruit set and the growth of pollen tubes in the pistil con firmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of‘Guiyou No.1'.Segregation ratios of the S genotype in progeny,expression analysis,and western blotting validated that the reduced levels of S_(2)-RNase mRNA contribute to the loss of SI in‘Guiyou No.1'.Furthermore,we report a phased assembly of the‘Guiyou No.1'pummelo genome and obtained two complete and well-annotated S haplotypes.Coupled with an analysis of SV variations,methylation levels,and gene expression,we identi fied a candidate gene(CgHB40),that may in fluence the regulation of the S/^RNase promoter.Our data provide evidence that a mutation that affects the pistilled to the loss of SI in‘Guiyou No.1'by in fluencing a poorly understood mechanism that affects transcriptional regulation.This work signi ficantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes. 关键词：CITRUS No.1 compatibility

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Down regulated expression of S_(2)-RNase attenuates self-incompatibility in “Guiyou No.1”pummelo

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