Gut Microbiota Community Shift with Severity of Coronary Artery Disease

查看全文 作　　者：Jia-Lu [1]Hu;Zhi-Feng [1]Yao;Min-Na [1]Tang;Chun [2]Tang;Xiao-Fan [2]Zhao;Xi [3]Su;Dan-Bo [1]Lu;Qiu-Rong [2]Li;Zhang-Sheng [4]Wang;Yan [1]Yan;Zeneng [5]Wang

机构地区：[1]Department of Cardiology,Zhongshan Hospital,Fudan University,Shanghai 200032,China;[2]Research Institute of General Surgery,Jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China;[3]Department of Laboratory Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China;[4]Department of Cardiology,The Fifth People’s Hospital of Shanghai,Fudan University,Shanghai 200240,China;[5]Department of Cardiovascular and Metabolic Sciences,Lerner Research Institute,Cleveland Clinic,Cleveland,OH 44195,USA

出　　处：《Engineering》索引2021年第7卷第12期,共10页

基　　金：This study was supported in part by the Shanghai Xuhui District Science and Technology Commission,Artificial Intelligence Project 2 for Jia-Lu Hu;Shanghai Public Health Talents Training Project(GWV-10.2-YQ11)for Jia-Lu Hu;the National Natural Science Foundation of China(81873538)for Yan Yan;the National Heart,Lung,and Blood Institute and the Office of the Director,National Institutes of Health(RO1HL130819)for Zeneng Wang. 摘　　要：Gut microbiota community shift with coronary artery disease(CAD)has been reported in several limited cohorts during the past several years.However,whether the enriched or decreased microbiota taxa with CAD can be reproducible deserves further investigation and validation.In this study,78 human subjects were recruited.Of these,19 were diagnosed without stenosis in coronary artery(control group,referred to herein as Ctrl),14 with stenosis less than 50%(LT50),and 45 with stenosis greater than 50%(GT50).Fecal samples were collected and DNA was extracted to perform 16S ribosomal RNA(rRNA)gene sequencing.The operational taxonomic units(OTUs)were analyzed to identify taxa specific to different groups;next,multivariate logistic regression was employed to test whether the defined taxa could independently predict CAD risk.We found that Deltaproteobacteria,Fusobacterium,Bilophila,Actinomyces,and Clostridium XIX were enriched in Ctrl;Prevotellaceae,Parabacteriodes,and Butyricicoccus were enriched in LT50;and Roseburia and Butyricimonas were enriched in GT50.Further analysis revealed that increased populations of Deltaproteobacteria,Fusobacterium,Bilophila,and Desulfovibrionaceae were associated with a 0.26-fold,0.21-fold,0.18-fold,and 0.26-fold decreased risk of CAD,respectively(p<0.05),and an increased Prevotellaceae population was associated with a 5.63-fold increased risk of CAD(p<0.01).A combination of the 20 microbial taxa achieved an area under the receiver operating characteristic(ROC)curve of higher than 0.88 for all discriminations between LT50 vs Ctrl,GT50 vs Ctrl,LT50+GT50 vs Ctrl,and GT50 vs Ctrl+LT50.However,the microbial taxa previously reported as enriched in CAD patients or healthy controls could not be observed in our cohort except for Bacteroides.In conclusion,CAD patients showed a different microbial taxa signature than the healthy controls.However,the non-reproducibility of the microbiota taxa enriched in CAD across different cohorts limits the use of this signature in early diagnosis and prevention.Only decreased Bacteroides abundance was found to be a reliable marker to indicate CAD progression. 关键词：Gut microbiota ATHEROSCLEROSIS Coronary artery disease

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Gut Microbiota Community Shift with Severity of Coronary Artery Disease

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