A G-type lectin receptor-like kinase regulates the perception of oomycete apoplastic expansin-like proteins

查看全文 作　　者：Lei [1]Pi;Zhiyuan [1,2]Yin;Weiwei [2]Duan;Nan [1]Wang;Yifan [1]Zhang;Jinghao [1]Wang;Daolong [1,2]Dou

机构地区：[1]College of Plant Protection,China Agricultural University,Beijing 100193,China;[2]College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Plant Biology》索引2022年第64卷第1期,共19页

基　　金：supported by the Fundamental Research Funds for the Central Universities(KYLH201703);the National Natural Science Foundation of China(32072507)。 摘　　要：Phytophthora capsici is one of the most harmful pathogens in agriculture, which threatens the safe production of multiple crops and causes serious economic losses worldwide. Here, we identified a P. capsici expansin-like protein, Pc EXLX1, by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with P. capsici. Clustered regularly interspaced short palindromic repeats/crispr associated protein9(CRISPR/Cas9)-mediated Pc EXLX1 knockout mutants exhibited significantly enhanced virulence,while the overexpression of Pc EXLX1 impaired the virulence. Prokaryotically expressed Pc EXLX1 activated multiple plant immune responses, which were BRI1-associated kinase 1(BAK1)-and suppressor of BIR1-1(SOBIR1)-dependent. Furthermore, overexpression of Pc EXLX1 homologs in N. benthamiana could also increase plant resistance to P. capsici. A G-type lectin receptor-like kinase from N. benthamiana, expansin-regulating kinase 1(ERK1), was shown to regulate the perception of Pc EXLX1 and positively mediate the plant resistance to P. capsici. These results reveal that the expansin-like protein, Pc EXLX1, is a novel apoplastic effector with plant immunity-inducing activity of oomycetes, perception of which is regulated by the receptor-like kinase, ERK1. 关键词：apoplastic effector ELICITOR Nicotiana benthamiana OOMYCETE receptor-like kinase

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A G-type lectin receptor-like kinase regulates the perception of oomycete apoplastic expansin-like proteins

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