RAMAN SPECTROSCOPY FOR IN VIVO TISSUE ANALYSIS AND DIAGNOSIS,FROM INSTRUMENT DEVELOPMENT TO CLINICAL APPLICATIONS

查看全文 作　　者：HAISHAN [1]ZENG;JIANHUA [1]ZHAO;MICHAEL [1]SHORT;DAVID [1]I.MCLEAN;STEPHEN [1]LAM;ANNETTE [1]MCWILLIAMS;HARVEY [1]LUI 机构地区：[1]Laboratory for Advanced Medical Photonics(LAMP)Cancer Imaging Department British Columbia Cancer Research Centre 675 West 10th Avenue Vancouver,BC,V5Z 1L3,Canada Photomedicine Institute Department of Dermatology and Skin Science University of British Columbia and Vancouver Coastal Health Research Institute 835 West 10th Avenue Vancouver,BC,V5Z 4E8,Canada 出　　处：《Journal of Innovative Optical Health Sciences》索引2008年第1卷第1期,共12页 基　　金：supported by the National Cancer Institute of Canada with funds from the Canadian Cancer Society,the Canadian Institutes of Health Research(Grant No.PPP-79109 and MOP-85011);the Canadian Dermatology Foundation,the VGH&UBC Hospital Foundation In It for Life Fund,and the BC Hydro Employees Community Services Fund. 摘　　要：Raman spectroscopy is a noninvasive,nondestructive analytical method capable of determining the biochemical constituents based on molecular vibrations.It does not require sample preparation or pretreatment.However,the use of Raman spectroscopy for in vivo clinical applications will depend on the feasibility of measuring Raman spectra in a relatively short time period(a few seconds).In this work,a fast dispersive-type nearinfrared(NIR)Raman spectroscopy system and a skin Raman probe were developed to facilitate real-time,noninvasive,in vivo human skin measurements.Spectrograph image aberration was corrected by a parabolic-line fiber array,permitting complete CCD vertical binning,thereby yielding a 16-fold improvement in signal-to-noise ratio.Good quality in vivo skin NIR Raman spectra free of interference from fiber fluorescence and silica Raman scattering can be acquired within one second,which greatly facilitates practical noninvasive tissue characterization and clinical diagnosis.Currently,we are conducting a large clinical study of various skin diseases in order to develop Raman spectroscopy into a useful tool for non-invasive skin cancer detection.Intermediate data analysis results are presented.Recently,we have also successfully developed a technically more challenging endoscopic Laser-Raman probe for early lung cancer detection.Preliminary in vivo results from endoscopic lung Raman measurements are discussed. 关 键 词：Rapid Raman system in vivo skin Raman in vivo lung Raman endoscopic Raman probe early cancer detection