Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β

查看全文 作　　者：LIN Kai-[1,2,3]li;ZHANG [4]Ji;CHUNG Hau-[2]lam;WU Xin-[2]yi;LIU [5]Bin;ZHAO Bo-[6]xin;SZE Stephen Cho-[2,3]wing;ZHOU Ping-[7]zheng;YUNG Ken Kin-[2,3]lam;ZHANG Shi-[2,3]qing

机构地区：[1]School of Public Health,Guangzhou Medical University,Guangzhou(511436),China;[2]Department of Biology,Hong Kong Baptist University(HKBU),Hong Kong Special Administrative Region(999077),China;[3]HKBU Shenzhen Research Institute and Continuing Education,Shenzhen,Guangdong Provinee(518057),China;[4]Department of Plastic Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou(510515),China;[5]Guangzhou Institute of Cardiovascular Disease,the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou(510260),China;[6]Department of Pharmacy,Nan fang Hospital,Souther n Medical Un iversity,Guan gzhou(510515),China;[7]Guangdong Provincial Key Laboratory of New Drug Screening,School of Pharmaceutical Sciences,Southern Medical University,Guangzhou(510515),China

出　　处：《Chinese Journal of Integrative Medicine》索引2022年第28卷第3期,共7页

基　　金：Supported by the National Natural Science Foundation of China(No.81703728)。 摘　　要：Objective:To study the effects of total ginsenosides(TG)extract from Panax ginseng on neural stem cell(NSC)proliferation and differentiation and their underlying mechanisms.Methods:The migration of NSCs after treatment with various concentrations of TG extract(50,100,or 200μg/mL)were monitored.The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays.NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2(MAP2).The GSK-3β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay.The NSCs with constitutively active GSK-3βmutant were made by adenovirus-mediated gene transfection,then the proliferation and differentiation of NSCs mediated by TG were further verified.Results:TG treatment significantly enhanced NSC migration(P<0.01 or P<0.05)and increased the proliferation of NSCs(P<0.01 or P<0.05).TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression(P<0.01 or P<0.05).TG extract also significantly induced GSK-3βphosphorylation at Ser9,leading to GSK-3βinactivation and,consequently,the activation of the GSK-3β/β-catenin pathway(P<0.01 or P<0.05).In addition,constitutive activation of GSK-3βin NSCs by the transfection of GSK-3βS9 A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation(P<0.01 or P<0.05).Conclusion:TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3β. 关键词：GINSENOSIDE neural stem cell PROLIFERATION neuronal differentiation GSK-3Β

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Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β

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