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Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics

查看全文 作　　者：Shihui [1,2]Qiu;Chuanjie [1,2]Shen;Xiaoyu [1]Jian;Yunxing [1,2]Lu;Zhaoduo [1,2]Tong;Zhenhua [1]Wu;Hongju [1,2]Mao;Jianlong [1,2]Zhao 高影响力作者 机构地区：[1]State Key State Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China;[2]Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Sciences,Beijing 100049,China高影响力机构 出　　处：《Chinese Chemical Letters》索引2022年第33卷第5期,共4页高影响力期刊 基　　金：supported by the National Key Research and Development Program of China(No.2017YFA0205300);National Natural Science Foundation of China(Nos.61971410,61701171,61801464,61801465 and 62001458);Shanghai Sailing Program(No.20YF1457100),Shanghai Engineer&Technology Research Center of Internet of Things for Respiratory Medicine(No.20DZ2254400);the Science and Technology Commission of Shanghai Munic-ipality(No.19511104200). 摘　　要：Tumor heterogeneity plays a critical role in the determination of appropriate anticancer therapy.As cir-culating tumor cells(CTCs)contain all tumor-related information,the genetic changes on CTCs could help us choose the appropriate treatments for different patients.Single-base mutations are very common in tumor genetic changes which may result in drug resistance.Here,we introduce a single-cell mutation de-tection platform based on droplet microfluidics.This platform integrates cell capsulation,cell lysis,poly-merase chain reaction(PCR)and the observation process.The droplets’generation speed is over 6000 per minute and more than 600 cells could be encapsulated in one second.To verify the performance of our platform in practical use,we performed the mutation analysis of 4 kinds of cells with our platform and noted that the genetic status of each single cell was clearly discriminated.Moreover,these results agreed with those from direct sequencing.Compared with other forms of single-cell mutation detection techniques,our platform has high throughput,short experimental time and less experimental operations. 关 键 词：Single cell CTCS Point mutation EGFR MICROFLUIDICS
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