A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

查看全文 作　　者：Yuan [1]Liu;Taotao [2]Li;Gaojian [1]Yang;Yan [3]Deng;Xianbo [4]Mou;Nongyue [1,3]He

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China;[2]Hunan Provincial Key Lab of Dark Tea and Jin-Hua,School of Materials and Chemical Engineering,Hunan City University,Yiyang 413000,China;[3]Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China;[4]Medical School of Ningbo University,Ningbo 315211,China

出　　处：《Chinese Chemical Letters》索引2022年第33卷第4期,共4页

基　　金：financially supported by the National Key Research and Development Program of China (No. 2018YFC1602905);the National Natural Science Foundation of China (Nos.61871180 and 61527806) for the financial supports。 摘　　要：In this work, a simple gold nanoparticles(Au NPs) based colorimetric biosensor was developed for chlorpyrifos(Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant(Kd) of 58.59 ± 6.08 nmol/L was selected by ss DNA library immobilized systematic evolution of ligands by enrichment(SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for Au NPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately,thus a self-aggregation of Au NPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection. 关键词：APTAMER Colorimetric aptasensor SELEX CHLORPYRIFOS AuNPs

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A simple AuNPs-based colorimetric aptasensor for chlorpyrifos detection

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