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A novel on-tissue cycloaddition reagent for mass spectrometry imaging of lipid C=C position isomers in biological tissues

查看全文 作　　者：Chenglong [1,2]Sun;Chunxia [1,2]Ma;Lili [1,2]Li;Yuhao [2]Han;Daijie [1,2]Wang;Xiao [1,2]Wan 高影响力作者 机构地区：[1]School of Pharmaceutical Sciences,Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250014,China;[2]Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250014,China高影响力机构 出　　处：《Chinese Chemical Letters》索引2022年第33卷第4期,共4页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China (Nos. 81903571, 21904080);the Taishan Scholars the Taishan Scholars Program (C. Sun, No. tsqn202103096);the Shandong Province “Double-Hundred Talent Plan” Program。 摘　　要：Application of matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI) to investigate the spatiotemporal alterations of lipids in biological tissues has brought many significant results.However, the presence of structural isomers varying in C=C double bond(DB) locations makes isomerresolved MSI an urgent need. Herein, we introduce a new type of light-driven on-tissue [2 + 2] cycloaddition reaction coupled with MALDI-MS/MS imaging to identify lipid DB position isomers and their spatial signatures in biological tissues. 3-Benzoylpyridine was introduced as a novel derivatization reagent, and it exhibited great reactivity toward lipid C=C bond to form oxetanes under both ultraviolet light and visible light irradiation. With this approach, DB position isomers of lipids were imaged with highly differential levels in distinct regions of rat brain, providing an accurate and spatially resolved approach to study tissue lipidomics. 关 键 词：MALDI-MSI On-tissue cycloaddition LIPID C=C position isomers Biological tissues
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