Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

查看全文 作　　者：Yu [1]Zhong;Yuwen [1]Wang;Baojian [2]Chen;Jinchu [1]Liu;Dong [1]Wang;Mengran [1]Li;Xiaolong [1]Qi;Chenxu [1]Liu;Kim [2]Boutilier;Shaojiang [1]Chen

机构地区：[1]National Maize Improvement Center of China,Key Laboratory of Crop Heterosis and Utilization/Engineering Research Center for Maize Breeding,Ministry of Educati on,College of Agronomy and Biotech no logy,China Agricultural Un iversity,Beijing 100193,China;[2]Bioscienee,Wageningen University and Research,6700 AA,Wageningen,The Netherlands

出　　处：《Journal of Integrative Plant Biology》索引2022年第64卷第6期,共14页

基　　金：supported by National Key Research and Development Program of China (2016YFD0101200, 2018YFD0100201);China Agriculture Research System of MOF and MARA, National Natural Science Foundation of China (91935303, 32001554);the 2020 Research Program of Sanya Yazhou Bay Science and Technology City (SKJC-2020-02-003);China Postdoctoral Science Foundation (2020TQ0356) 摘　　要：Doubled haploid(DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction(HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid(allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP-HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops. 关键词：amphihaploid Brassica napus dicot crops doubled haploid tech no logy maternal haploid in ducti on Nicotiana tabacum POLYPLOID

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Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

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