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Effect of environmental stresses on the survival and cytotoxicity of Shiga toxin-producing Escherichia coli

查看全文 作　　者：Brian Byongkwon [1,3]Yoo;Yanhong [2]Liu;Vijay [1]Juneja;Lihan [1]Huang;Cheng-An [1]Hwang 高影响力作者 机构地区：[1]Residue Chemistry and Predictive Microbiology Research Unit and;[2]Molecular Characterization of Foodborne Pathogens Research Unit,Eastern Regional Research Center,Agricultural Research Service,United States Department of Agriculture,Wyndmoor,Pennsylvania,USA;[3]Present address:Clinical and Environmental Microbiology Branch,Division of Healthcare Quality and Promotion,National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention,1600 Clifton Rd.NE,Atlanta,GA 30329,USA高影响力机构 出　　处：《Food Quality and Safety》索引2017年第1卷第2期,共8页高影响力期刊 摘　　要：The objective of this study was to determine the effect of environmental stresses on the cytotoxicity of Shiga toxin-producing Escherichia coli (STEC). STEC O157:H7 and six non-O157 STEC strains (O26:H11, O103:H1, O104:H4, O111:NM, O121:NM, and O145:NM) were subjected to osmotic (aw 0.95-0.98), acid (pH 4-7), and chlorine (1-5 ppm) stresses. After stress treatments, bacterial populations, expression of virulence-associated genes, and Vero-cytotoxicity were determined. Among the strains, O145:NM survived at aw 0.97 longer than other serotypes, while O111:NM was significantly more sensitive to osmotic stress. At pH 4, O103:H1 was more resistant to the stress, while O26:H11 and O111:NM had significantly less growth. For 2 ppm chlorine stress, O26:H11, O103:H1, and O145:NM had higher populations (>3 log) than other strains. Stressed strains showed a significant increase in relative gene expression levels of stx1, stx2, and eae in O103:H1, O104:H4, and O145:NM than non-stressed control. Additionally, significantly higher Vero-cytotoxicity, as indicated by lactate dehydrogenase assay, of stressed O26:H11, O103:H1, O104:H4, and O145:NM was observed. The results suggest that the growth and cytotoxicity of selected pathogenic E. coli may be enhanced after being exposed to environmental stresses. 关 键 词：Pathogenic Escherichia coli STRESS GROWTH virulence genes Vero-cytotoxicity.
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