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Biodiversity of fungi on Vitis vinifera L. revealed by traditional and high-resolution culture-independent approaches

查看全文 作　　者：Ruvishika [1,2,3]S.Jayawardena;Witoon [4]Purahong;Wei [1,3]Zhang;Tesfaye [4,5]Wubet;XingHong [1,3]Li;Mei [1,3]Liu;Wensheng [6]Zhao;Kevin [2]D.Hyde;JianHua [1]Liu;Jiye [1,3]Yan 高影响力作者 机构地区：[1]Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,People’s Republic of China;[2]Centre of Excellence in Fungal Research,Mae Fah Luang University,Chiang Rai 57100,Thailand;[3]Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China,Beijing 100097,People’s Republic of China;[4]Department of Soil Ecology,UFZ-Helmholtz Centre for Environmental Research,Theodor-Lieser-Str.4,06120 Halle(Saale),Germany;[5]German Centre for Integrative Biodiversity Research(iDiv)Halle-Jena-Leipzig,Leipzig,Germany;[6]College of Agricultural and Biotechnology,China Agricultural University,Beijing 100193,People’s Republic of China高影响力机构 出　　处：《Fungal Diversity》索引2018年第3期,共84页高影响力期刊 基　　金：s This work was financially supported by Beijing Talent Programm for Jiye Yan,CARS-29 and JNKYT201605. 摘　　要：This study is unique as it compares traditional and high-resolution culture-independent approaches using the same set of samples to study the saprotrophic fungi on Vitis vinifera.We identified the saprotrophic communities of table grape(Red Globe)and wine grape(Carbanate Gernischet)in China using both traditional and culture-independent techniques.The traditional approach used direct observations based on morphology,single spore isolation and phylogenetic analysis yielding 45 taxa which 19 were commonly detected in both cultivars.The same set of samples were then used for Illumina sequencing which analyzed ITS1 sequence data and detected 226 fungal OTUs,of which 176 and 189 belong to the cultivars Carbanate Gernischet and Red Globe,respectively.There were 139 OTUs shared between the two V.vinifera cultivars and 37 and 50 OTUs were specific to Carbanate Gernischet and Red Globe cultivars respectively.In the Carbanate Gernischet cultivar,Ascomycota accounted for 77%of the OTUs and in Red Globe,almost all sequenced were Ascomycota.The fungal taxa overlap at the genus and species level between the traditional and culture-independent approach was relatively low.In the traditional approach we were able to identify the taxa to species level,while in the culture-independent method we were frequently able to identify the taxa to family or genus level.This is remarkable as we used the same set of samples collected in China for both approaches.We recommend the use of traditional techniques to accurately identify taxa.Culture-independent method can be used to get a better understanding about the organisms that are present in a host in its natural environment.We identified primary and secondary plant pathogens and endophytes in the saprotrophic fungal communities,which support previous observations,that dead plant material in grape vineyards can be the primary sources of disease.Finally,based on present and previous findings,we provide a worldwide checklist of 905 fungal taxa on Vitis species,which includes their mode of life and distribution. 关 键 词：CHECKLIST GRAPEVINE Mycobiome Next generation sequencing PATHOGENS Saprotrophs
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