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An innovative method used for the identification of N-glycans on soybean allergenβ-conglycinins

查看全文 作　　者：Cheng [1]Li;Yang [1]Tian;Jianli [2]Han;Yu [2]Lu;Meiyi [2]Zou;Yue [2]Jia;Chengjian [1]Wang;Linjuan [1,2]Huang;Zhongfu [1,2]Wang 高影响力作者 机构地区：[1]Key Laboratory of Glycobiology and Glycoengineering of Xi’an,College of Food Science and Technology,Northwest University,Xi’an 710069,China;[2]College of Life Sciences,Northwest University,Xi’an 710069,China高影响力机构 出　　处：《Food Science and Human Wellness》索引2023年第12卷第3期,共9页高影响力期刊 基　　金：funded by National Natural Science Foundation of China(31870798,31972024);Shaanxi Province Innovation Capability Support Plan-Science and Technology Innovation Team(2020TD-044);Key Laboratory of Glycobiology and Glycoengineering of Xi’an(2019219514SYS010CG032);Natural Science Project of Shaanxi Provincial Department of Education(21JK0929);Science and Technology Resources Sharing Platform Project of Science and Technology Department in Shaanxi Province(2022PT-46)。 摘　　要：β-Conglycinin is one of the major allergens existed in soybean.N-Glycans attached to theβ-conglycinin influenced the immunoreactivity and antigen presenting efficiency ofβ-conglycinin.In this study,we described a new method used to release and collect the N-glycans fromβ-conglycinin,and the N-glycans existed in linear epitopes ofβ-conglycinin were identified.Glycopeptides hydrolyzed fromβ-conglycinin were purified by cotton hydrophilic chromatography.Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides,and new glycopeptides containing one single N-acety1-D-glucosamine(G1 cNAc)moiety were then utilized for mass spectrometry.Five glycosylation sites(Asn-199,Asn-455,Asn-215,Asn-489 and Asn-326)and 22 kinds of glycopeptides were identified.It is noteworthy that the peptide VVN^(#)ATSNL(where^(#)represents for the glycosylation site)was analyzed to be both glycopeptide and linear epitope.Our results provided a new method for the N-glycoform analysis of food allergens,and laid a foundation for understanding the relationship between glyco sylation and food allergy. 关 键 词：Soybean allergenβ-conglycinin GLYCOPEPTIDE Mass spectrometry N-GLYCAN Glycosylation site
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