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Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE

查看全文 作　　者：Jianping [1,2]Kang;Jieyi [1,2]Li;Zhou [1,2]Guo;Sijie [1,2]Zhou;Shuxin [1,2]Su;Wenhai [1,2]Xiao;Yi [1,2]Wu;Yingjin [1,2]Yuan 高影响力作者 机构地区：[1]Frontier Science Center for Synthetic Biology(Ministry of Education),Tianjin University,Tianjin,300072,China;[2]Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,China高影响力机构 出　　处：《Synthetic and Systems Biotechnology》索引2022年第7卷第3期,共9页高影响力期刊 基　　金：supported by the National Key R&D Program of China(2021YFC2102500);National Natural Science Foundation of China(31971351). 摘　　要：Varied environmental stress can affect cell growth and activity of the cellular catalyst.Traditional path of adaptive evolution generally takes a long time to achieve a tolerance phenotype,meanwhile,it is a challenge to dissect the underlying genetic mechanism.Here,using SCRaMbLE,a genome scale tool to generate random structural variations,a total of 222 evolved yeast strains with enhanced environmental tolerances were obtained in haploid or diploid yeasts containing six synthetic chromosomes.Whole genome sequencing of the evolved strains revealed that these strains generated different structural variants.Notably,by phenotypic-genotypic analysis of the SCRaMbLEd strains,we find that a deletion of gene YFR009W(GCN20)can improve salt tolerance of Saccharomyces cerevisiae,and a deletion of gene YER056C can improve 5-flucytosine tolerance of Saccharomyces cerevisiae.This study shows applications of SCRaMbLE to accelerate phenotypic evolution for varied environmental stress and to explore relationships between structural variations and evolved phenotypes. 关 键 词：SCRAMBLE Saccharomyces cerevisiae Structural variation GCN20 TOLERANCE
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