维普中文期刊产品整合服务

Bioinformatic Analysis and Experimental Verification of QJHGD on Caerulein-induced Inflammatory Response in SAP Model Rats Based on TLR4/NF-κB/My D88 Pathway

查看全文 作　　者：Baijun [1]QIN;Xiping [2]TANG;Xin [1]YANG;Xianzhong [1]BU;Wenhao [1]GONG;Yueqiao [3]CHEN;Guozhong [3]CHEN 高影响力作者 机构地区：[1]Guangxi University of Chinese Medicine,Nanning 530001,China;[2]The Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,China;[3]The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China高影响力机构 出　　处：《Medicinal Plant》索引2022年第13卷第4期,共9页高影响力期刊 基　　金：Supported by Project of National Natural Science Foundation of China(8216150526);Natural Scienceof Guangxi(2020GXNSFAA297062);SAP Early TCM and Western Medicine Treatment Program of Guangxi Zhuang Autonomous Region Promotion and Application Project(S2019021);Project of Guangxi Graduate Education Innovation(YCBXJ2021010&YCBXJ2021009)。 摘　　要：[Objectives]To conduct bioinformatic analysis and experimental verification of Qingjie Huagong Decoction(QJHGD)on caerulein-induced inflammatory response in severe acute pancreatitis(SAP)model rats based on TLR4/NF-κB/MyD88 pathway.[Methods]The effective component groups and potential targets of QJHGD were collected by the network pharmacology method.A drug-component-target network was constructed.The GO and KEGG of targets were enriched and analyzed with the aid of Metascape database,and the target pathway related to SAP inflammation was screened.The SAP rat model was established by caerulein combined with lipopolysaccharide,and QJHGD was intragastrically administered.Pancreatic tissue was observed by HE staining.In addition,enzyme-linked immunosorbent assay and immunohistochemistry were used to verify the anti-inflammatory effect of QJHGD on SAP rats and its regulatory effect on TLR4/NF-κB/MyD88 target pathway.[Results]A total of 105 active components of QJHGD and 148 key targets of SAP were predicted and screened;KEGG was enriched in 320 different pathways including toll-like receptor and NF-κB classical pathways.Animal experiment verified that QJHGD reduced serum amylase,serum lipase activity,IL-6,TNF-αlevels in SAP rats;HE staining showed the effect of QJHGD on the pathological changes of pancreas,and QJHGD inhibited the positive expression of key proteins of TLR4,NF-κB and MyD88 in the inflammatory transduction pathway.[Conclusions]The mechanism of QJHGD improving pancreatic injury in SAP rats may be related to down-regulating the expression of key proteins in the TLR4/NF-κB/MyD88 pathway. 关 键 词：TLR4/NF-κB/MyD88 pathway Severe acute pancreatitis(SAP) Qingjie Huagong Decoction(QJHGD) Inflammatory response Network pharmacology Experimental verification
相关文献

参考文献(18)

耦合文献(549)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费