Direct-acting Antiviral-induced Transient Recovery of NK Cells in Early-stage Treatment of Chronic Hepatitis C Patients

查看全文 作　　者：Xiaohui [1,2]Zhang;Yingying [3]Jiang;Shaobin [4]Li;Dandan [5]Bian;Mei [6]Liu;Ming [1,2]Kong;Yu [1,2]Chen;Zhongping [1,2]Duan;Sujun [2,7]Zheng

机构地区：[1]The Fourth Department of Hepatology,Beijing YouAn Hospital,Capital Medical University,Beijing,China;[2]Beijing Municipal Key Laboratory of Liver Failure and Artificial Liver Treatment&Research,Beijing YouAn Hospital,Capital Medical Univer-sity,Beijing,China;[3]Department of Gastroenterology and Hepatology,Beijing YouAn Hospital,Capital Medical University,Beijing,China;[4]School of Energy and Power Engineering,Beihang University,Beijing,China;[5]Department of Infectious Diseases,Electric Power Teaching Hospital,Capital Medical University,Beijing,China;[6]Oncology Department,Beijing YouAn Hospital,Capital Medical University,Beijing,China;[7]The First Department of Hepatology,Beijing YouAn Hospital,Capital Medical University,Beijing,China

出　　处：《Journal of Clinical and Translational Hepatology》索引2022年第10卷第6期,共8页

基　　金：Beijing Advanced Innova-tion Center for Big Data-based Precision Medicine(No.PXM2021_014226_000026);Special key research pro-ject of capital health development scientific research(No.SF2021-1G-2181);National Natural Science Foundation(No.81500472);Beijing Natural Science Foundation(No.7202070);Beijing Municipal Administration of Hospitals Clinical medicine Development of special funding support(No.ZYLX202125). 摘　　要：Background and Aims:The rapid clearance of hepatitis C virus induced by direct-acting antivirals(DAAs)affects natural killer(NK)cells,but the reported results are not consistent,and the relative mechanism was unclear.This study focused on the dynamic changes of NK cells during and after DAA treatment and analyzed the reasons.Meth-ods:Peripheral blood from 35 chronic hepatitis C patients who were treated with DAAs were collected at baseline and weeks 1,2,4,12,and post-treatment week-12.The fre-quency,subset,and phenotype of NK cells were assayed by flow cytometry.Lactate dehydrogenase assays were used to evaluate the cytotoxicity of NK cells.Cytokine concentra-tions were measured with Luminex kits.Results:All pa-tients achieved a sustained viral response(SVR),and the NK cell frequencies were not changed significantly during DAA therapy.However,the cytotoxicity of NK cells recov-ered significantly early in week 1,and then continuously decreased below normal levels.The changes of genotypes including NKp30+,NKp46+,and NKG2A+NK cells were par-allel to NK function.The subset of CD56dim NK cells con-tinuously increased and did not return to normal even at 12 weeks after treatment.Interleukin(IL)-2,IL10,IL15,interferon-gamma,and tumor necrosis factor-alpha all in-creased after week 4,peaked at the end of therapy,and then exhibited varying degrees of reduction with time.Con-clusions:DAA treatment led to transient functional recov-ery of NK cells in the early stage of treatment,and then continuously decreased to below normal levels.Alterations of NK subsets,phenotypes,and the microenvironment may be involved in the changes. 关键词：Hepatitis C Natural killer cell CYTOKINES PHENOTYPE

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Direct-acting Antiviral-induced Transient Recovery of NK Cells in Early-stage Treatment of Chronic Hepatitis C Patients

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