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Calcium/calmodulin modulates salt responses by binding a novel interacting protein SAMS1 in peanut(Arachis hypogaea L.)

查看全文 作　　者：Sha [1]Yang;Jianguo [1]Wang;Zhaohui [1]Tang;Yan [2]Li;Jialei [1]Zhang;Feng [1]Guo;Jingjing [1]Meng;Feng [1]Cui;Xinguo [1]Li;Shubo [3]Wan 高影响力作者 机构地区：[1]Institute of Crop Germplasm Resources,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China;[2]Institute of Agricultural Resources and Environment,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China;[3]Shandong Academy of Agricultural Sciences/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,Shandong,China高影响力机构 出　　处：《The Crop Journal》索引2023年第11卷第1期,共12页高影响力期刊 基　　金：supported by the National Key Technology Research and Development Program of China (2018YFD1000900);the Natural Science Foundation of Shandong Province(ZR2020MC094);the Natural Science Foundation of Shandong Province (ZR2021QC163);Special Funds for Local Science and Technology Development Guided by the Central Committee(YDZX20203700001861)。 摘　　要：The Ca^(2+)/CaM signal transduction pathway helps plants adapt to environmental stress. However, our knowledge on the functional proteins of C^(2+)/CaM pathway in peanut(Arachis hypogeae L.) remains limited. In the present study, a novel calmodulin 4(CaM4)-binding protein S-adenosyl-methionine synthetase 1(SAMS1) in peanut was identified using a yeast two-hybrid assay. Expression of AhSAMS1was induced by Ca^(2+), ABA, and salt stress. To elucidate the function of AhSAMS1, physiological and phenotypic analyses were performed with wild-type and transgenic materials. Overexpression of AhSAMS1increased spermidine and spermidine synthesis while decreased the contents of ethylene, thereby eliminating excessive reactive oxygen species(ROS) in transgenic lines under salt stress. AhSAMS1 reduced uptake of Na+and leakage of K+from mesophyll cells, and was less sensitive to salt stress during early seedling growth, in agreement with the induction of SOS and NHX genes Transcriptomics combined with epigenetic regulation uncovered relationships between differentially expressed genes and differentially methylated regions, which raised the salt tolerance and plants growth. Our findings support a model in which the role of AhSAMS1 in the ROS-dependent regulation of ion homeostasis was enhanced by Ca^(2+)/CaM while AhSAMS1-induced methylation was regulated by CaM, thus providing a new strategy for increasing the tolerance of plants to salt stress. 关 键 词：AhCaM4 AhSAMS1 Protein interaction Polyamines Salt tolerance
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