HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley

查看全文 作　　者：Deshui [1,2,3]Yu;Renchun [2]Fan;Ling [2]Zhang;Pengya [2,3]Xue;Libing [1]Liao;Meizhen [2]Hu;Yanjun [2]Cheng;Jine [2,3]Li;Ting [2]Qi;Shaojuan [2]Jing;Qiuyun [2]Wang;Arvind [1]Bhatt;Qian-Hua [2,3]Shen

机构地区：[1]Lushan Botanical Garden,Chinese Academy of Science,Jiujiang 332900,Jiangxi,China;[2]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Innovation Academy for Seed Design,Beijing 100101,China;[3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《The Crop Journal》索引2023年第11卷第1期,共9页

基　　金：supported by National Key Research and Development Program of China(2018YFD1000703,2018YFD1000700);Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11020400);National Program on Research and Development of Transgenic Plants(2016ZX08009-003-001);Startup Fund for Advanced Talents of Lushan Botanical Garden;Chinese Academy of Science(2020ZWZX03 and 2020ZWZX05);the“Double Hundred and Double Thousand”Talent Project of Jiujiang City(jjsbsq2020026)。 摘　　要：Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner.In barley,Hv WRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus,Blumeria graminis f.sp.hordei(Bgh).However,the molecular features of Hv WRKY2 in its DNA-binding and repressor functions,as well as its target genes,are uncharacterized.We show that the W-box binding of Hv WRKY2 requires an intact WRKY domain and an upstream sequence of~75 amino acids,and the Hv WRKY2 W-box binding activity is linked to its repressor function in disease resistance.Chromatin immunoprecipitation(ChIP)-seq analysis identified HvCEBiP,a putative chitin receptor gene,as a target gene of Hv WRKY2 in overexpressing transgenic barley plants.ChIP-qPCR and Electrophoretic Mobility Shift Assay(EMSA)verified the direct binding of Hv WRKY2 to a W-boxcontaining sequence in the HvCEBiP promoter.Hv CEBiP positively regulates resistance against Bgh in barley.Our findings suggest that Hv WRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern(PAMP)recognition receptor genes,suggesting that Hv CEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection. 关键词：HvWRKY2 Basal immunity Chromatin immunoprecipitation(ChIP) HvCEBiP

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HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley

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