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Salivary microbiota may predict the presence of esophageal squamous cell carcinoma

查看全文 作　　者：Junmin [1,2]Wei;Ruifeng [2,3]Li;Yanxian [1,2]Lu;Fan [4]Meng;Bohong [1,2]Xian;Xiaorong [5]Lai;Xiayi [8]Lin;Yu [8]Deng;Dongyang [5]Yang;Huabin [8]Zhang;Liangfang [6]Li;Xiaosong [7]Ben;Guibin [7]Qiao;Wanwei [6]Liu;Zijun [1,2,6]Li 高影响力作者 机构地区：[1]Guangdong Provincial Institute of Geriatrics,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,PR China;[2]The Second School of Clinical Medicine,Southern Medical University,Guangzhou,Guangdong 510515,PR China;[3]Wuhan No.1 Hospital,Wuhan,Hubei 430022,PR China;[4]The First Affiliated Hospital,Gannan Medical University,Ganzhou,Jiangxi 341000,PR China;[5]Medicine-Oncology,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,PR China;[6]Department of Gastroenterology,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,PR China;[7]Department of Thoracic Surgery,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,PR China;[8]Concord Medical Center,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong 510080,PR China高影响力机构 出　　处：《Genes & Diseases》索引2022年第9卷第4期,共9页高影响力期刊 基　　金：This study was supported by the Scientific Research Project of Guangzhou, Guangdong Province, China (No. 20184010458). 摘　　要：The aim is to explore the predictive value of salivary bacteria for the presence of esophageal squamous cell carcinoma (ESCC). Saliva samples were obtained from 178 patients with ESCC and 101 healthy controls, and allocated to screening and verification cohorts, respectively. In the screening phase, after saliva DNA was extracted, 16S rRNA V4 regions of salivary bacteria were amplified by polymerase chain reaction (PCR) with high-throughput sequencing. Highly expressed target bacteria were screened by Operational Taxonomic Units clustering, species annotation and microbial diversity assessment. In the verification phase, the expression levels of target bacteria identified in the screening phase were verified by absolute quantitative PCR (Q-PCR). Receiver operating characteristic (ROC) curves were plotted to investigate the predictive value of target salivary bacteria. LEfSe analysis revealed higher proportions of Fusobacterium, Streptococcus and Porphyromonas, and Q-PCR assay showed significantly higher numbers of Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in patients with ESCC, when compared with healthy controls (all P < 0.05). The areas under the ROC curves for Streptococcus salivarius, Fusobacterium nucleatum, Porphyromonas gingivalis and the combination of the three bacteria for predicting patients with ESCC were 69%, 56.5%, 61.8% and 76.4%, respectively. The sensitivities corresponding to cutoff value were 69.3%, 22.7%, 35.2% and 86.4%, respectively, and the matched specificity were 78.4%, 96.1%, 90.2% and 58.8%, respectively. These highly expressed Streptococcus salivarius, Fusobacterium nucleatum and Porphyromonas gingivalis in the saliva, alone or in combination, indicate their predictive value for ESCC. 关 键 词：Esophageal squamous cell carcinoma(ESCC) Predictive value Quantitative polymerase chain reaction(Q-PCR) Receiver operating characteristic(ROC)curves Salivary bacteria
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