Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic

查看全文 作　　者：Wei [1]Tian;Lingdi [1]Liu;Zhongxing [2]Song;Zhishu [2]Tang;Chunxiu [1]Wen;Tao [1]Jiang 机构地区：[1]Institute of Cash Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang,050051,China;[2]State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi University of Chinese Medicine,Xianyang,712046,China 出　　处：《Phyton-International Journal of Experimental Botany》索引2023年第92卷第2期,共14页 基　　金：supported by Hebei Province Key Research and Development Program Special Project(22326301D);Ministry of Science and Technology Project(2017YFC1700702);HAAFS Agriculture Science and Technology Innovation Project(2022KJCXZX-JZS-4);Technical System of Traditional Chinese Medicine Industry in Hebei Province(HBCT2018060203);State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),No.QY202102. 摘　　要：The extract from Semen Ziziphi Spinosae(SZS)contains many secondary metabolites(flavonoids,alkaloids,and terpenoids),widely reported for their high medicinal value.In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS,we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic.A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages(T1,T2,and T3)of SZS.The main flavonoid metabolic components of SZS were catechin,L-epicatechin,(–)-epigallocatechin,(+)-gallocatechin,spinosin and its derivatives.A total of 53 unigenes encoding 15 enzymes were identified as candidate genes involved in flavonoid biosynthesis in SZS.Among,PAL,C4H,4CL,CHS,CHI,FLS,FNS,ANS,ANR,LAR,and UFGT genes were all downregulated in T3 than in T1.The expression levels of these genes influence the accumulation of flavonoids in SZS.Our results should provide valuable information for flavonoid metabolites and candidate genes involved in flavonoid biosynthesis on SZS . 关 键 词：Spinosin secondary metabolites TRANSCRIPTOMIC METABOLOMIC gene expression