Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus

查看全文 作　　者：Liang [1,2]Chai;Cheng [1]Cui;Benchuan [1]Zheng;Jinfang [1]Zhang;Jun [1]Jiang;Haojie [1,2]Li;Liangcai [1]Jiang 机构地区：[1]Crop Science Institute,Sichuan Academy of Agricultural Sciences,Chengdu,610066,China;[2]Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province,Chengdu,610066,China 出　　处：《Phyton-International Journal of Experimental Botany》索引2023年第92卷第3期,共19页 基　　金：funded by Modern Agro-Industry Technology Research System of China(CARS-12);Independent Innovation Project of SAAS(2022ZZCX004);1+9 Open Competition Project of SAAS(1+9KJGG002,1+9KJGG001);the Accurate Identification Project of Crop Germplasm from Sichuan Provincial Finance Department;Sichuan Science and Technology Program(2022ZDZX0015);Sichuan Crop Breeding Community(2021YFYZ0018);Disciplinary Construction Project for Modern Agriculture in SAAS(2021XKJS003);Chengdu Science and Technology Project(2021-YF09-00062-SN). 摘　　要：Nitrogen(N)is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field.Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive.In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed(Brassica napus L.),comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root,stem and leaf under different N concentrations,respectively.More than 200 differential abundance proteins(DAPs)were detected and categorized into groups according to annotations,including“binding and catalytic activity”,“involved in primary metabolism and cellular processes”,“stress-response”and so on.Variation in chlorophyll(Chl)content and antioxidant activities further revealed that oxidative stress raised with the increase of N concentration.Bioinformatics analysis based on the expression level of total proteins suggested these DAPs might play important roles in adaptation to N-stress conditions.Generally,these results provides a new aspect into N-stress responding proteins in Brassica plants. 关 键 词：Brassica napus nitrogen(N)response oxidative stress PROTEOMICS