Cowpea NAC1/NAC2 transcription factors improve growth and tolerance to drought and heat in transgenic cowpea through combined activation of photosynthetic and antioxidant mechanisms

查看全文 作　　者：Richa [1]Srivastava;Yuriko [2]Kobayashi;Hiroyuki [2]Koyama;Lingaraj [1]Sahoo

机构地区：[1]Department of Biosciences and Bioengineering Indian Institute of Technology Guwahati Guwahati,Assam 781039 India;[2]Faculty of Applied Biological Sciences Gifu University 1-1,Yanagido,Gifu 501-1193,Japan

出　　处：《Journal of Integrative Plant Biology》索引2023年第65卷第1期,共20页

基　　金：supported by a research grant from the Program Support Grant Phase-II from the Department of Biotechnology,Government of India to L.S. (BT/PR13560/COE/34/44/2015)。 摘　　要：NAC(NAM/ATAF1/2/CUC2)transcription factors are central switches of growth and stress responses in plants.However,unpredictable interspecies conservation of function and regulatory targets makes the well-studied NAC orthologs inapt for pulse engineering.The knowledge of suitable NAC candidates in hardy pulses like cowpea(Vigna unguiculata(L.)Walp.)is still in infancy,hence warrants immediate biotechnological intervention.Here,we showed that overexpression of two native NAC genes(VuNAC1and VuNAC2)promoted germinative,vegetative,and reproductive growth and conferred multiple abiotic stress tolerance in a commercial cowpea variety.The transgenic lines displayed increased leaf area,thicker stem,nodule-rich denser root system,early flowering,higher pod production(~3.2-fold and~2.1-fold),and greater seed weight(10.3%and 6.0%).In contrast,transient suppression of VuNAC1/2 caused severe growth retardation and flower inhibition.The overexpressor lines showed remarkable tolerance to major yielddeclining terminal stresses,such as drought,salinity,heat,and cold,and recovered growth and seed production by boosting photosynthetic activity,water use efficiency,membrane integrity,Na^(+)/K^(+)homeostasis,and antioxidant activity.The comparative transcriptome study indicated consolidated activation of genes involved in chloroplast development,photosynthetic complexes,cell division and expansion,cell wall biogenesis,nutrient uptake and metabolism,stress response,abscisic acid,and auxin signaling.Unlike their orthologs,VuNAC1/2 direct synergistic transcriptional tuning of stress and developmental signaling to avoid unwanted trade-offs.Their overexpression governs the favorable interplay of photosynthesis and reactive oxygen species regulation to improve stress recovery,nutritional sufficiency,biomass,and production.This unconventional balance of strong stress tolerance and agronomic quality is useful for translational crop research and molecular breeding of pulses. 关键词：COWPEA enhanced photosynthetic activity improved growth improved yield legume genetic engineering multiple stress tolerance NAC transcription factor VuNAC

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Cowpea NAC1/NAC2 transcription factors improve growth and tolerance to drought and heat in transgenic cowpea through combined activation of photosynthetic and antioxidant mechanisms

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