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PuHox52 promotes coordinated uptake of nitrate,phosphate, and iron under nitrogen deficiency in Populus ussuriensis

查看全文 作　　者：Ming [1,2]Wei;Mengqiu [1]Zhang;Jiali [1]Sun;Ying [1]Zhao;Solme [1]Pak;Miaomiao [1]Ma;Yingxi [1]Chen;Han [2]Lu;Jingli [1]Yang;Hairong [3]Wei;Yuhua [2,4]Li;Chenghao [1]Li 高影响力作者 机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,School of Forestry,Northeast Forestry University,Harbin 150040,China;[2]College of Life Sciences,Northeast Forestry University,Harbin 150040,China;[3]College of Forest Resources and Environmental Science,Michigan Technological University,Houghton,Michigan 49931,USA;[4]Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University),Ministry of Education,Harbin 150040,China高影响力机构 出　　处：《Journal of Integrative Plant Biology》索引2023年第65卷第3期,共19页高影响力期刊 基　　金：supported by the NSFC (31971671);the Fundamental Research Funds for the Central Universities of China (2572018CL04);the China Postdoctoral Science Foundation (2021M700733);the Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)。 摘　　要：It is of great importance to better understand how trees regulate nitrogen(N) uptake under N deficiency conditions which severely challenge afforestation practices, yet the underlying molecular mechanisms have not been well elucidated. Here,we functionally characterized PuHox52, a Populus ussuriensis HD-ZIP transcription factor, whose overexpression greatly enhanced nutrient uptake and plant growth under N deficiency. We first conducted an RNA sequencing experiment to obtain root transcriptome using PuHox52-overexpression lines of P. ussuriensis under low N treatment. We then performed multiple genetic and phenotypic analyses to identify key target genes of PuHox52 and validated how they acted against N deficiency under PuHox52 regulation.PuHox52 was specifically induced in roots by N deficiency, and overexpression of PuHox52promoted N uptake, plant growth, and root development. We demonstrated that several nitrate-responsive genes(PuNRT1.1, PuNRT2.4,PuCLC-b, PuNIA2, PuNIR1, and PuNLP1),phosphate-responsive genes(PuPHL1A and PuPHL1B), and an iron transporter gene(PuIRT1) were substantiated to be direct targets of PuHox52. Among them, PuNRT1.1, PuPHL1A/B, and PuIRT1 were upregulated to relatively higher levels during PuHox52-mediated responses against N deficiency in PuHox52-overexpression lines compared to WT. Our study revealed a novel regulatory mechanism underlying root adaption to N deficiency where PuHox52 modulated a coordinated uptake of nitrate, phosphate, and iron through 'PuHox52-PuNRT1.1', 'PuHox52-PuPHL1A/PuPHL1B', and'PuHox52-PuIRT1' regulatory relationships in poplar roots. 关 键 词：HD-Zip transcription factor iron transport nitrate transport nitrogen deprivation phosphate absorption Populus ussuriensis root architecture target gene
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