Identification of gut microbes associated with feed efficiency by daily-phase feeding strategy in growing-finishing pigs

查看全文 作　　者：Qin [1,2,3]Jiang;Chunlin [1,2,3]Xie;Lingli [1,2,3]Chen;Hongli [1,2,3]Xiao;Zhilian [1,2,3]Xie;Xiaoyan [1,2,3]Zhu;Libao [1,2,3]Ma;Xianghua [1,2,3]Yan 机构地区：[1]State Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Frontiers Science Center for Animal Breeding and Sustainable Production,College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China;[2]The Cooperative Innovation Center of Sustainable Pig Production,Wuhan,Hubei 430070,China;[3]Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology,Wuhan,Hubei 430070,China 出　　处：《Animal Nutrition》索引2023年第1期,共12页 基　　金：This study was financially supported by National Natural Science Foundation of China(31925037,320021190);China Postdoctoral Science Foundation funded project(2019TQ0108). 摘　　要：Feed efficiency is one of the most important issues for sustainable pig production.Daily-phase feeding(DPF)is a form of precision feeding that could improve feed efficiency in pigs.Gut microbiota can regulate host nutrient digestion,absorption,and metabolism.However,which key microbes may play a vital role in improving the feed efficiency during DPF remains unclear.In the present study,we used a DPF program compared to a three-phase feeding(TPF)program in growing-finishing pigs to investigate the effects of gut microbiota on feed efficiency.A total of 204 Landrace×Yorkshire pigs(75 d)were randomly assigned into 2 treatments.Each treatment was replicated 8 times with 13 to 15 pigs per replicate pen.Pigs in the TPF group were fed with a commercial feeding program that supplied fixed feed for phasesⅠ,Ⅱ,andⅢ,starting at 81,101,and 132 d of age,respectively,and pigs in the DPF group were fed a blend of adjacent phase feed from 81 to 155 d at a gradual daily ratio and phaseⅢfeed from 155 to 180 d of age.Daily feed intake and body weight were recorded by a computerized device in the feeders.Feces and blood samples were collected from 1 pig per replicate at 155 and 180 d of age.The results showed that the DPF program remarkably improved the feed efficiency at 155 d(P<0.001)and 180 d of age(P<0.001),with a significant reduction of the intake of crude protein(P<0.01),net energy(P<0.001),crude fiber(P<0.001),ether extract(P<0.01),and ash(P<0.001).The daily-phase feeding program increased the abundance of Prevotella copri(P<0.05)and Paraprevotella clara(P<0.05),while it decreased the abundance of Ocilibacter(P<0.05)at 155 d of age.The results of correlation analysis indicated that the differentially abundant microbiota communities were closely associated with 20 metabolites which enriched amino acid and phenylalanine metabolism.Our results suggest that 2 key microbes may contribute to feed efficiency during daily-phase feeding strategies in pigs. 关 键 词：Daily-phase feeding Feed efficiency Gut microbiota Nutrient metabolism PIG