pH-responsive aldehyde-bearing cyclometalated iridium(Ⅲ) complex for tracking intracellular pH fluctuations under external stimulation

查看全文 作　　者：Manping [1]Qian;Danyang [1]Zhang;Hetong [2]Qi;Xuebing [3]Yang;Guanjun [3]Yin;Chengxiao [1]Zhang;Jianzhong [3]Guo;Honglan [1]Qi 机构地区：[1]Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi'an 710062,China;[2]School of Chemistry,Xi'an Jiaotong University,Xi'an 710049,China;[3]Shaanxi Province Key Laboratory of Ultrasound,School of Physics and Information Technology,Shaanxi Normal University,Xi'an 710062,China 出　　处：《Chinese Chemical Letters》索引2023年第34卷第2期,共5页 基　　金：supported by the National Natural Science Foundation of China (Nos. 22074087, 21804106 and 11727813);the Fundamental Research Funds for the Central Universities (No. 2021TS032)。 摘　　要：Intracellular p H undertakes critical functions in various biological and pathological processes. It is important to monitor intracellular p H fluctuations for understanding physiological and pathological processes.Here, one aldehyde-bearing cyclometalated iridium(Ⅲ) complex([(4-pba)_(2)Ir(dcphen)]PF_(6), 4-pba = 4-(2-pyridyl) benzaldehyde, dcphen = 4,7-dichloro-1,10-phenanthroline, probe 1) was synthesized and used to track intracellular p H fluctuations. Probe 1 displayed p H-dependent luminescence property in p H range of 1.81–6.81 with an evaluated p Kavalue of 4.30 in BR buffer-DMSO(v:v = 99:1). An intramolecular hydrogen bonds assisted p H-responsive mechanism was proposed for the p H-responsive behavior of probe1. Probe 1 was successfully applied for imaging and tracking p H fluctuations in He La cells under external stimulation with fast response time, good photostability as well as low cytotoxicity and high cell permeability. This work demonstrates that aldehyde-bearing cyclometalated iridium(Ⅲ) complex can be used as alternative p H-responsive probe for real-time tracking intracellular p H fluctuations, which provides a strategy for the design of p H-responsive probe in versatile applications. 关 键 词：Cyclometalated iridium(Ⅲ)complex Photoluminescence pH Intramolecular hydrogen bonds External stimulation