Yes-associated protein contributes to magnesium alloy-derivedinflammation in endothelial cells

查看全文 作　　者：Hongchi [1,2]Yu;Zhe [1]Hou;Nuoya [1]Chen;Rifang [1]Luo;Li [1]Yang;Michael [3]Miao;Xiaoyi [4]Ma;Lifeng [4]Zhou;Fugui [4]He;Yang [2]Shen;Xiaoheng [2]Liu;Yunbing [1]Wang

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610065,China;[2]Institute of Biomedical Engineering,West China School of Basic Medical Sciences&Forensic Medicine,Sichuan University,Chengdu 610041,China;[3]Division of Oral&Craniofacial Health Sciences,University of North Carolina Adams School of Dentistry,Chapel Hill,NC 27599,USA;[4]Beijing Key Laboratory of Cardiac Drug Device Technology and Evidence Based Medicine,Beijing 100021,China

出　　处：《Regenerative Biomaterials》索引2022年第9卷第1期,共13页

基　　金：supported by the National Natural Science Foundation of China(11802190);National Key Research and Development Program(2016YFC1102200);the 111 Project The Program of Introducing Talents of Discipline to Universities(B16033). 摘　　要：Magnesium alloy(Mg alloy)has attracted massive attention in the potential applications of cardiovascular stents because of its good biocompatibility and degradability.However,whether and how the Mg alloy induces inflammation in endothelial cells remains unclear.In the present work,we investigated the activation of Yes-associated protein(YAP)upon Mg alloy stimuli and unveiled the transcriptional function in Mg alloy-induced inflammation.Quantitative RT–PCR,western blotting and immunofluorescence staining showed that Mg alloy inhibited the Hippo pathway to facilitate nuclear shuttling and activation of YAP in human coronary artery endothelial cells(HCAECs).Chromatin immunoprecipitation followed sequencing was carried out to explore the transcriptional function of YAP in Mg alloy-derived inflammation.This led to the observation that nuclear YAP further bonded to the promoter region of inflammation transcription factors and co-transcription factors.This binding event activated their transcription and modified mRNA methylation of inflammation-related genes through regulating the expression of N6-methyladenosine modulators(METTL3,METTL14,FTO and WTAP).This then promoted inflammation-related gene expression and aggravated inflammation in HCAECs.In YAP deficiency cells,Mg alloy-induced inflammation was reduced.Collectively,our data suggest that YAP contributes to the Mg alloy-derived inflammation in HCAECs and may provide a potential therapeutic target that alleviates inflammation after Mg alloy stent implantation. 关键词：magnesium alloy Yes-associated protein INFLAMMATION

相关文献

参考文献（42）
引证文献（1）
耦合文献（0）

参考文献(42)

引证文献(1)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Yes-associated protein contributes to magnesium alloy-derivedinflammation in endothelial cells

参考文献(42)

引证文献(1)

用户提示