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Cell osteogenic bioactivity mediated precisely by varying scaled micro-pits on ordered micro/nano hierarchical structures of titanium

查看全文 作　　者：Yanmei [1]Zhang;Xiankuan [1]Wang;Yaxian [1]Li;Jianhe [1]Liang;Pinliang [1]Jiang;Qiaoling [2]Huang;Yun [2]Yang;Hongping [3]Duan;Xiang [3]Dong;Gang [4]Rui;Changjian [1,5]Lin 高影响力作者 机构地区：[1]State Key Laboratory of Physical Chemistry of Solid Surfaces,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;[2]Research Institute for Biomimetics and Soft Matter,Fujian Provincial Key Laboratory for Soft Functional Materials Research,College of Physical Science and Technology,Xiamen University,Xiamen 361005,China;[3]Beijing Engineering Laboratory of Functional Medical Materials and Devices,Beijing Medical Implant Engineering Research Center,Beijing Naton Technology Group Co.Ltd,Beijing,China;[4]Department of Orthopedics Surgery,The First Affiliated Hospital of Xiamen University,Xiamen,Fujian 361003,China;[5]Tan Kah Kee Innovation Laboratory,Xiamen,Fujian 361005,China高影响力机构 出　　处：《Regenerative Biomaterials》索引2022年第9卷第1期,共15页高影响力期刊 基　　金：financial supports from the National Natural Science Foundation of China(grant no.21773199,51571169,21621091);the State Key Project of Research and Development(grant no.2016YFC1100301). 摘　　要：Hierarchical surface structures with micro–nano scale play a crucial role in regulation of cell proliferation and osteogenic differentiation.It has been proven that cells are extremely sensitive to the nanoscaled structure and show multifarious phenotypes.Though a vital function of microstructure on osseointegration has been confirmed,the cell performances response to different microscaled structure is needed to be further dissected and in depth understood.In this work,the ordered micro–nano hierarchical structures with varying micro-scaled pits were precisely fabricated on titanium successfully by the combination of electrochemical,chemical etching and anodization as well.In vitro systematical assessments indicated that the micro–nano multilevel structures on titanium exhibited excellent cells adhesion and spreading ability,as well as steerable proliferation and osteogenic differentiation behaviors.It is shown that smaller micro-pits and lower roughness of the hierarchical structures enabled faster cell propagation.Despite cell growth was delayed on micro–nano titanium with relatively larger cell-match-size micro-pits and roughness,osteogenic-specific genes were significantly elevated.Furthermore,the alkaline phosphatase activity,collagen secretion and extracellular matrix mineralization of MC3T3-E1 on multiscaled titanium were suppressed by a large margin after adding IWP-2(an inhibitor of Wnt/b-catenin signal pathway),indicating this pathway played a crucial part in cell osteogenic differentiation modulated by micro–nano structures. 关 键 词：electrochemical self-organizing etching varying micro-scaled pits OSTEOGENIC Wnt/b-catenin
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