维普中文期刊产品整合服务

Cryogenic superresolution correlative light and electron microscopy of vitreous sections

查看全文 作　　者：Buyun [1,2]Tian;Maoge [1]Zhou;Fengping [1]Feng;Xiaojun [1]Xu;Pei [1,2]Wang;Huiqin [3]Luan;Wei [1,2]Ji;Yanhong [1]Xue;Tao [1,2]Xu 高影响力作者 机构地区：[1]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China;[2]University of Chinese Academy of Sciences,Beijing 100049,China;[3]National Research Center for Rehabilitation Technical Aids,Beijing 100176,China高影响力机构 出　　处：《Biophysics Reports》索引2022年第8卷第4期,共12页高影响力期刊 基　　金：funded by the National Key Research and Development Program of China(2017YFA0504700);the National Natural Science Foundation of China(32027901)。 摘　　要：Fluorescence microscopy and electron microscopy complement each other as the former provides labelling and localisation of specific molecules and target structures while the latter possesses excellent revolving power of fine structure in context.These two techniques can combine as correlative light and electron microscopy(CLEM)to reveal the organisation of materials within the cell.Frozen hydrated sections allow microscopic observations of cellular components in situ in a near-native state and are compatible with superresolution fluorescence microscopy and electron tomography if sufficient hardware and software support is available and a well-designed protocol is followed.The development of superresolution fluorescence microscopy greatly increases the precision of fluorescence annotation of electron tomograms.Here,we provide detailed instructions on how to perform cryogenic superresolution CLEM on vitreous sections.From fluorescence-labelled cells to high pressure freezing,cryoultramicrotomy,cryogenic single-molecule localisation microscopy,cryogenic electron tomography and image registration,electron tomograms with features of interest highlighted by superresolution fluorescence signals are expected to be obtained. 关 键 词：CRYOGENIC SUPERRESOLUTION Fluorescence microscopy Electron microscopy Correlative light and electron microscopy(CLEM) Electron tomography
相关文献

参考文献(35)

引证文献(1)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费