维普中文期刊产品整合服务

BmCH25H,a vertebrate interferon-stimulated gene(ISG)homolog,inhibits BmNPV infection dependent on its hydroxylase activity in Bombyx mori

查看全文 作　　者：Hongyun [1]Wu;Junming [1]Xia;Shigang [1]Fei;Yeyuan [1]Wang;Mengmeng [1]Zhang;Yiyao [1]Guo;Xian [1]Li;Luc [2]Swevers;Jingchen [1]Sun;Min [1]Feng 高影响力作者 机构地区：[1]Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou,China;[2]Insect Molecular Genetics and Biotechnology,National Center for Scientific Research Demokritos,Institute of Biosciences and Applications,Athens,Greece高影响力机构 出　　处：《Insect Science》索引2023年第30卷第2期,共17页高影响力期刊 基　　金：supported by the Natural Science Foundation of Guangdong Basic and Applied Basic Research Fund(2022A1515012657);Guangzhou Science and Technology Plan Project(202002030218);National Natural Science Foundation of China(31872426);South China Agricultural University high-level talent launch project;Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry(No.2018-143);Innovation and Entrepreneurship Training Program for College Students in Guangdong Province(S202110564098). 摘　　要：Cholesterol-25-hydroxylase(CH25H)has been identified as an interferon-stimulated gene(ISG)in mammals that exerts its antiviral effects by catalyzing the conversion of cholesterol to 25-hydroxycholesterol(25HC).However,invertebrates lack an antiviral system homologous to vertebrate interferons(IFNs)because the genomes of invertebrates do not encode IFN-like cytokines.Nevertheless,CH25H is present in insect genomes and it therefore deserves further study of whether and by which mechanism it could exert an antiviral effect in invertebrates.In this study,the Bombyx mori CH25H(BmCH25H)gene,of which the encoded protein has high homology with other lepidopteran species,was identified and located on chromosome 9.Interestingly,we found that the expression of BmCH25H was significantly upregulated in B.mori nucleopolyhedrovirus(BmNPV)-infected BmN cells and silkworm(B.mori)larvae at the early infection stage.The inhibitory effect of BmCH25H on BmNPV replication was further demonstrated to depend on its catalytic residues to convert cholesterol to 25HC.More importantly,we demonstrated that during BmNPV infection,BmCH25H expression was increased through the Janus kinase–signal transducer and activator of transcription(JAK–STAT)pathway,similar to the induction of ISGs following virus infection in vertebrates.This is the first report that CH25H has antiviral effects in insects;the study also elucidates the regulation of its expression and its mechanism of action. 关 键 词：Bombyx mori nucleopolyhedrovirus Bombyx mori Cholesterol 25-hydroxylase Interferon-stimulated genes Signal transducer and activator of transcription
相关文献

参考文献(47)

引证文献(4)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费