In-situ formation of“electron conductive wires”threaded ZIF-8 membrane for multiplexed immunoassay of human interleukins

查看全文 作　　者：Yu [1]Lv;Mingshi [1]Deng;Xuefeng [2]Wang;Xinghua [1]Gao;Lingyan [1]Feng;Wei [3]Chen;Chung-Chiun [4]Liu;Xinxin [2]Li;Yuan [1]Zhang 机构地区：[1]Materials Genome Institute,Shanghai University,Shanghai 200444,China;[2]State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China;[3]Department of Emergency,Tongji Hospital,Tongji University School of Medicine,Shanghai 200065,China;[4]Department of Chemical and Biomolecular Engineering,Case Western Reserve University,Cleveland,OH 44106 出　　处：《Nano Research》索引2023年第16卷第2期,共9页 基　　金：funded by the National Key Research and Development Program of China(No.2021YFB3200804);Shanghai“Road and Belt”International Cooperation Project(No.19520744200);the Key Basic Research Program of Science and Technology Commission of Shanghai Municipality(No.20JC1415303). 摘　　要：To address the challenge of highly sensitive and accurate detection of biomarkers in complex environments,a rational engineering strategy for designing electrochemical immunosensing platform is proposed.Herein,we develop a microsensor chip through the combination of multiplexed electrodes and microfluidic channels for the parallel detection of human interleukins(IL-6 and IL-8).For the construction of an efficient sensing interface,the conductive silver nanowires(Ag NWs)wrapped with zeolitic imidazolate framework(ZIF-8)thin film(denoted as ZIF-8@Ag NWs)are prepared,and then employed for the multi-functionalization of electrodes.The immunodetection of ILs is based on the direct signal transduction ability of Ag NWs and specific interaction of periodically arranged Zn2+ions in ZIF-8 films with biomolecules,which offer the high assay sensitivity and good specificity.The immunosensor chip achieves a wide detection range from pg/mL to ng/mL and possesses the ability to resist non-specific proteins adsorption in biological complex media.Further clinical serum samples assay verifies that the combination of IL-6 and IL-8 levels yields high diagnostic accuracy.Principal component analysis(PCA)reveals that 18 patient samples could be fully separated from healthy control samples.The low-cost,easily fabricated electrochemical immunosensing platform provides a rapid serum test for diagnosis and personalized therapy of inflammatory diseases,and can also be generalized to other immunoreaction-based biomarkers detections. 关 键 词：zeolitic imidazolate framework(ZIF) integrated nanostructure MICROSENSOR biomarker detection INTERLEUKINS