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Protein trap-engineered metal-organic frameworks for advanced enzyme encapsulation and mimicking

查看全文 作　　者：Weiqing [1]Xu;Yu [1]Wu;Lei [1]Jiao;Meng [1]Sha;Xiaoli [1]Cai;Yating [1]Wen;Yifeng [1]Chen;Wenling [1]Gu;Chengzhou [1]Zhu 高影响力作者 机构地区：[1]Key Laboratory of Pesticide and Chemical Biology of Ministry of Education,International Joint Research Center for Intelligent Biosensing Technology and Health,College of Chemistry Central China Normal University,Wuhan 430079,China高影响力机构 出　　处：《Nano Research》索引2023年第16卷第2期,共8页高影响力期刊 基　　金：support of the National Natural Science Foundation of China(Nos.22074049 and 22004042);the Fundamental Research Funds for the Central Universities(No.CCNU22JC006);the Program of Introducing Talents of Discipline to Universities of China(111 program,No.B17019). 摘　　要：Immobilizing enzymes within metal-organic frameworks(MOFs)enables enzymes to against extreme environments.However,these MOF shells are just like armors,protective but heavy,which shield the enzymes from threats while locking them in the cage.The exploitation of immobilization strategy and intrinsic property of MOFs themselves is of great significance.Here,we proposed a functional protein trap strategy for efficient enzyme encapsulation.The ferrocenedicarboxylic acid(Fc)was used to induce the formation of defect-rich Co-based MOFs(CoBDC-Fc).As result,the engineered protein trap can not only improve the enzyme loading but also accelerate catalytic efficiency.Specifically,the atomically dispersed Fc sites serve as cocatalysts/cofactors and even change the conformation of enzymes in the construed microenvironment.Furthermore,the obtained CoBDC-Fc/enzyme exhibits excellent recyclability and tolerance to inhospitable conditions.Benefited by these,the CoBDC-Fc/enzyme/antigen composites were further prepared for cascade enzyme-linked immunosorbent assay of prostate-specific antigen with satisfactory sensitivity. 关 键 词：enzyme immobilization metal-organic frameworks BIOCATALYSIS nanozymes IMMUNOASSAY
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