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A compact liquid chromatography-mass spectrometry instrument for the quantitation of immunosuppressants

查看全文 作　　者：Hao [1]Gu;Guoxin [1]Dai;Siyu [1]Liu;Yanbing [2]Zhai;Qian [1,3]Xu;Hongqiang [4]Wu;Wei [1]Xu 高影响力作者 机构地区：[1]School of Life Science,Beijing Institute of Technology,Beijing 100081,China;[2]School of Medical Technology,Beijing Institute of Technology,Beijing 100081,China;[3]National Institute of Metrology,Beijing 100029,China;[4]Biotree Metabolomics Technology Research Center,Shanghai 201800,China高影响力机构 出　　处：《Chinese Chemical Letters》索引2023年第34卷第4期,共5页高影响力期刊 基　　金：supported by the National Key Research and Development Program of China (No. 2020YFF01014502);the National Natural Science of Foundation of China (Nos. 21922401,201827810)。 摘　　要：Liquid chromatography tandem mass spectrometry(LC-MS/MS) plays an important role in clinical diagnostics. Although LC-MS/MS is superior in terms of accurately quantifying molecules in complex matrices,instrument footprint, operation and maintenance complexity also hinder its expansion as the analytical technique of choice. In this study, a compact LC-MS instrument was developed, in which an assembled liquid chromatograph was coupled with a miniature ion trap mass spectrometer. The overall instrument has a footprint of 69 cm × 31 cm × 31 cm, and it requires no gas supply as well as minimum maintenance. Furthermore, the use of LC-MS is in accord with conventional clinical diagnostic protocols, and the choice of ion trap offers tandem MS performance. The results showed that the use of LC could improve both mixture analysis capability and detection sensitivity of the miniature mass spectrometer. After optimization, feasibility of this instrument in clinical practice was demonstrated by the quantitation of four widely used immunosuppressants in blood samples. Relatively good linearities were obtained, which spanned the reference ranges of effective therapeutic concentrations of each immunosuppressant. Intraday and inter-day accuracy and precision of analytical method were also assessed. This work showed that a compact LC-MS instrument could be used in clinical diagnosis, either to replace conventional lab-scale instruments or to be used in POCT applications. 关 键 词：Mass spectrometry INSTRUMENTATION LC-MS IMMUNOSUPPRESSANTS
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